Department of Electrical and Computer Engineering, North Dakota State University, Fargo, ND, USA.
Biomedical Engineering Program, North Dakota State University, Fargo, ND, USA.
Electrophoresis. 2021 May;42(9-10):1060-1069. doi: 10.1002/elps.202000347. Epub 2021 Feb 5.
The detection and quantification of nucleic acid and proteomic biomarkers in bodily fluids is a critical part of many medical screening and diagnoses. However, majority of the current detection platforms are not ideal for routine, rapid, and low-cost testing in point-of-care settings. To address this issue, we developed a concept for a disposable universal point-of-care biosensor that can detect and quantify nucleic acid and proteomic biomarkers in diluted serum samples. The central tenet of sensing is the use of dielectrophoresis, electrothermal effects, and thermophoresis to selectively and rapidly isolate the biomarkers of interest in electrodes and then quantify using electrical impedance. When the sensor was applied to quantify microRNA and antigen biomarker molecules directly in diluted serum samples, it produced a LOD values in the fM range and sensitivity values from 10 to 10 Ω/M with a 30 min assay time and assay cost of less than $50 per assay.
体液中核酸和蛋白质生物标志物的检测和定量是许多医学筛查和诊断的关键部分。然而，目前大多数检测平台并不适合在即时护理环境中进行常规、快速和低成本的检测。为了解决这个问题，我们开发了一种概念，即一种可一次性使用的通用即时护理生物传感器，该传感器可以检测和定量稀释血清样本中的核酸和蛋白质生物标志物。传感的核心原则是使用介电泳、电热效应和热泳来选择性和快速地在电极中分离感兴趣的生物标志物，然后使用电阻抗进行定量。当该传感器用于直接定量稀释血清样本中的 microRNA 和抗原生物标志物分子时，它在 30 分钟的检测时间和每份检测不到 50 美元的检测成本下，产生了 fM 范围内的 LOD 值和 10 到 10 Ω/M 的灵敏度值。
