Department of Biotechnology and Bioengineering, İzmir Institute of Technology, 35430, İzmir, Turkey.
Department of Agricultural Biotechnology, Tekirdağ Namık Kemal University, 59030, Tekirdağ, Turkey.
Appl Biochem Biotechnol. 2021 May;193(5):1574-1584. doi: 10.1007/s12010-021-03512-0. Epub 2021 Jan 28.
Proteases account for approximately 60% of the enzyme market in the world, and they are used in various industrial applications including the detergent industry. In this study, production and characterization of a novel serine protease of thermophilic Geobacillus sp. GS53 from Balçova geothermal region, İzmir, Turkey, were performed. The thermostable protease was purified through ammonium sulfate precipitation and anion-exchange chromatography. The results showed that the protease had 137.8 U mg of specific activity and optimally worked at 55 C and pH 8. It was also active in a broad pH (4-10) and temperature (25-75 °C) ranges. The protease was highly stable at 85 °C and demonstrated relative stability at pH 4, 7, and 10. Also, the enzyme had high stability against organic solvents and surfactants; enzyme relative activity did not decrease below 81% upon preincubation for 10 min. Ca, Cu, and Zn ions slightly induced protease activity. The protease was highly specific to casein, skim milk, Hammerstein casein, and BSA substrates. These results revealed that the protease might have a potential effect in a variety of industrial fields, especially the detergent industry, because of its high thermostability and stability to surfactants.
蛋白酶约占全球酶市场的 60%，广泛应用于各种工业领域，包括洗涤剂行业。本研究从土耳其伊兹密尔巴尔乔瓦地热区的嗜热芽孢杆菌 GS53 中生产和表征了一种新型丝氨酸蛋白酶。该耐热蛋白酶通过硫酸铵沉淀和阴离子交换层析进行纯化。结果表明，该蛋白酶的比活为 137.8 U mg，最适作用温度为 55°C，最适 pH 为 8。它在较宽的 pH（4-10）和温度（25-75°C）范围内均具有活性。该蛋白酶在 85°C 下高度稳定，在 pH 4、7 和 10 下表现出相对稳定性。此外，该酶对有机溶剂和表面活性剂具有较高的稳定性；在 10 分钟的预孵育后，酶的相对活性没有降低到 81%以下。Ca、Cu 和 Zn 离子轻微诱导蛋白酶的活性。该蛋白酶对酪蛋白、脱脂乳、Hammerstein 酪蛋白和 BSA 底物具有高度特异性。这些结果表明，由于其高耐热性和对表面活性剂的稳定性，该蛋白酶可能在各种工业领域，特别是洗涤剂行业具有潜在的应用效果。
