阿尔及利亚沙漠沙中嗜热菌株的蛋白质组学与分子综合鉴定及其酶学潜力

Integrated Proteomic and Molecular Identification of Thermophilic Strains from Algerian Desert Sands and Their Enzymatic Potential.

作者信息

Hammadi Amaria Ilhem, Merzoug Mohamed, Aireche Marwa, Zater Zohra Yasmine, Bendida Keltoum, Brakna Chaimaa Naila, Choubane Slimane, Todorov Svetoslav Dimitrov, Saidi Djamal

机构信息

Higher School of Biological Sciences of Oran, BP 1042 Saim Mohamed, Cité Emir Abdelkader (EX-INESSMO), Oran 31000, Algeria.

Laboratory of Biology of Microorganisms and Biotechnology, University of Oran1 Ahmed Ben Bella, Oran 31005, Algeria.

出版信息

Life (Basel). 2025 Aug 21;15(8):1327. doi: 10.3390/life15081327.

DOI:10.3390/life15081327

PMID:40868974

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12387256/

Abstract

Thermophilic microorganisms are among the key natural sources of thermostable enzymes, found not only in geothermal areas but also in arid environments. In this study, eight strains were isolated from the arid sands of Aïn Sefra (Naâma, Algeria) and characterized both phenotypically and genetically. All strains exhibited an optimal growth temperature of 70 °C, with most showing alkaliphilic pH preferences. Proteomic and molecular analyses (MALDI-TOF MS, 16S rRNA) identified as predominant, with BOX-PCR and RAPD-PCR revealing notable intraspecies diversity. All strains synthesized at least one thermostable enzyme (protease, amylase, laccase, or DNase) at their optimal temperature (70 °C), positioning them as promising candidates for biotechnological processes requiring extreme thermal conditions.

摘要

嗜热微生物是热稳定酶的关键天然来源之一，不仅存在于地热区域，也存在于干旱环境中。在本研究中，从艾因塞夫拉（阿尔及利亚纳马省）的干旱沙地中分离出8株菌株，并对其进行了表型和基因特征分析。所有菌株的最佳生长温度均为70°C，大多数菌株表现出嗜碱的pH偏好。蛋白质组学和分子分析（基质辅助激光解吸电离飞行时间质谱、16S rRNA）确定了主要菌株，BOX-PCR和RAPD-PCR揭示了显著的种内多样性。所有菌株在其最佳温度（70°C）下至少合成一种热稳定酶（蛋白酶、淀粉酶酶、漆酶或脱氧核糖核酸酶），使其成为需要极端热条件的生物技术过程的有前景的候选菌株。