Department of Oral and Maxillofacial Surgery, School of Dentistry, International Campus, Tehran University of Medical Sciences, Tehran, Iran.
Craniomaxillofacial Research Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Asian Pac J Cancer Prev. 2021 Jan 1;22(1):157-161. doi: 10.31557/APJCP.2021.22.1.157.
Despite the notable advances in modern surgery and radiotherapy,no significant increase in the five year survival rate of oral squamous cell carcinoma has been reported. Collecting evidence demonstrates that miR 153 and miR 455-5p play a key role in growth and progression of oral cancer. Early detection of OSCC is important for enhancing patient quality of life and clinical treatment.For this reason, biomarkers or tumour markers offer an opportunity to intervene and avoid development of oral cancer.
A total of 50 blood samples from patients from both genders (25 OSCC and 25 healthy people/control groups) were obtained to determine the expression of miR153 and miR455-5p using Real time Polymerase chain reaction and t test.
In general by using the formula Δ ct, it is evident that the miR 153 expression in peripheral blood is lower in patients than in healthy individuals (1.97) while the miR 455-5p expression in peripheral blood is higher in patients than in healthy individuals (2.56).
We conclude that miR153 and miR 455-5p expression in serum can function as a diagnostic screening test for the early detection of oral squamous cell carcinoma.
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尽管现代手术和放疗取得了显著进展，但口腔鳞状细胞癌的五年生存率并没有显著提高。证据表明，miR153 和 miR455-5p 在口腔癌的生长和进展中发挥关键作用。早期发现口腔癌对于提高患者生活质量和临床治疗非常重要。因此，生物标志物或肿瘤标志物提供了一个干预和避免口腔癌发展的机会。
共采集了 50 名男女患者（25 名口腔鳞状细胞癌患者和 25 名健康人/对照组）的血液样本，使用实时聚合酶链反应和 t 检验检测 miR153 和 miR455-5p 的表达。
一般来说，通过使用公式 Δ ct，外周血中 miR153 的表达在患者中低于健康人（1.97），而外周血中 miR455-5p 的表达在患者中高于健康人（2.56）。
我们得出结论，血清中 miR153 和 miR455-5p 的表达可以作为口腔鳞状细胞癌早期检测的诊断筛查试验。
