Guangzhou City Key Laboratory of Subtropical Fruit Trees Outbreak Control, Zhongkai University of Agriculture and Engineering, Guangzhou, China.
Guangzhou City Key Laboratory of Subtropical Fruit Trees Outbreak Control, Zhongkai University of Agriculture and Engineering, Guangzhou, China.
J Invertebr Pathol. 2021 Feb;179:107539. doi: 10.1016/j.jip.2021.107539. Epub 2021 Jan 25.
Asian citrus psyllid (ACP), Diaphorina citri Kuwayama (Hemiptera: Liviidae), is a serious pest of citrus. The insect also transmits Candidatus Liberibacter asiaticus, the pathogen of a devastating citrus disease called Huanglongbing. Clonostachys rosea is a versatile fungus that possesses nematicidal and insecticidal activities. The effect of C. rosea against D. citri remains unclear. Here we examined the pathogenicity of C. rosea against D. citri adults. A mortality rate of 46.67% was observed in adults treated with 1 × 10 conidia/mL spore suspension. Comparative transcriptomic analyses identified 259 differentially-expressed genes (DEGs) between controls and samples treated with fungi. Among the DEGs, 183 were up-regulated and 76 down-regulated. Genes with altered expression included those involved in immunity, apoptosis and cuticle formation. Our preliminary observation indicated that C. rosea is virulent against ACP adults and has the potential as a biological control agent for ACP management in the field.
亚洲柑橘木虱（ACP），柑橘臀纹粉蚧（Hemiptera：Liviidae），是柑橘的严重害虫。该昆虫还传播黄龙病菌，这是一种毁灭性的柑橘疾病的病原体。淡紫拟青霉是一种多功能真菌，具有杀线虫和杀虫活性。淡紫拟青霉对柑橘臀纹粉蚧的作用尚不清楚。在这里，我们研究了淡紫拟青霉对柑橘臀纹粉蚧成虫的致病性。用 1×10 个孢子/mL 孢子悬浮液处理的成虫死亡率为 46.67%。比较转录组分析鉴定出对照组和真菌处理组之间的 259 个差异表达基因（DEGs）。在 DEGs 中，183 个上调，76 个下调。表达发生改变的基因包括参与免疫、细胞凋亡和角质层形成的基因。我们的初步观察表明，淡紫拟青霉对 ACP 成虫具有毒力，并有可能作为田间 ACP 管理的生物防治剂。
