Guangzhou City Key Laboratory of Subtropical Fruit Trees Outbreak Control, Zhongkai University of Agriculture and Engineering, Guangzhou, China.
Guangzhou City Key Laboratory of Subtropical Fruit Trees Outbreak Control, Zhongkai University of Agriculture and Engineering, Guangzhou, China.
Gene. 2020 Feb 15;727:144246. doi: 10.1016/j.gene.2019.144246. Epub 2019 Nov 21.
The Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Liviidae) is a serious pest that feeds on plant phloem sap of citrus trees, and transmits Candidatus Liberibacter asiaticus, a bacterium that induces the destructive disease called Huanglongbing. Increasing evidence suggests that high temperatures could affect various biological traits, including size, longevity, mortality, behavior and metabolism of D. citri. However, the relevant mechanisms of heat stress remain unclear. In this study, a large set of transcriptomic data derived from D. citri adults were generated and differentially expressed genes (DEGs) after heat stress were identified by RNA sequencing. A total of 118, 399 unigenes were obtained, from which 37, 665 were mapped to sequences from at least one database. Seven hundreds and twenty-two unigenes were affected by high temperature of 40 °C for 4 h, in which 486 up-regulated and 236 down-regulated, and part of heat shock proteins, antioxidant and detoxification genes and cathepsins were identified as the DEGs. KEGG pathway enrichment analysis demonstrated that part of genes involved in protein synthesis and processing, metabolism, immunity, and signal transduction were differentially expressed under heat stress. Furthermore, the mRNA expression levels of 20 DEGs were confirmed by qRT-PCR, which verified the accuracy of high-throughput sequencing. Our results revealed that the response of D. citri adults to high temperatures is associated with a range of changes involved in various physiological and biochemical processes. Our data provide a basis for future research to improve our understanding on the molecular mechanism for heat responses in D. citri adults.
亚洲柑橘木虱 Diaphorina citri Kuwayama（半翅目：丽木虱科）是一种严重的害虫，以柑橘树韧皮部汁液为食，并传播导致黄龙病的韧皮部杆菌 Ca. Liberibacter asiaticus。越来越多的证据表明，高温可能会影响各种生物学特性，包括大小、寿命、死亡率、行为和柑橘木虱的新陈代谢。然而，热应激的相关机制仍不清楚。在这项研究中，通过 RNA 测序生成了大量源自柑橘木虱成虫的转录组数据，并鉴定了热应激后的差异表达基因（DEGs）。共获得 118399 条 unigenes，其中 37665 条被映射到至少一个数据库的序列。722 条 unigenes受到 40°C 高温 4 小时的影响，其中 486 条上调，236 条下调，部分热休克蛋白、抗氧化和解毒基因和组织蛋白酶被鉴定为 DEGs。KEGG 通路富集分析表明，部分参与蛋白质合成和加工、代谢、免疫和信号转导的基因在热应激下差异表达。此外，通过 qRT-PCR 验证了 20 个 DEG 的 mRNA 表达水平，这验证了高通量测序的准确性。我们的研究结果表明，柑橘木虱成虫对高温的反应与涉及各种生理和生化过程的一系列变化有关。我们的数据为未来的研究提供了基础，以提高我们对柑橘木虱成虫热反应分子机制的理解。
