Laboratory of Characterization and Valorization of Natural Resources, University Mohamed El Bachir El Ibrahimi, Bordj Bou-Arreridj, 34000, Algeria.
Department of Biology, Faculty of Nature and Life Sciences, University Bordj Bou-Arreridj, 34000, Algeria.
J Ethnopharmacol. 2021 May 23;272:113940. doi: 10.1016/j.jep.2021.113940. Epub 2021 Feb 22.
Salvia species are known to have anti-inflammatory properties, and are traditionally used for healing purposes. Salvia verbenaca is an Algerian plant used for healing wounds and ulcers.
This work aims to assess the acute and subacute safety of S. verbenaca and its possible anti-inflammatory activity as a mechanism contributing to its traditional applications.
Lethal toxicity of S. verbenaca hydromethanolic extract was evaluated against Artemia salina larvae, while acute and subacute toxicity were orally tested on mice. The anti-inflammatory activity was screened ex vivo using membrane stabilization and in vivo using xylene induced ear edema as an acute inflammation model. The antiradical, reducing power and iron chelating activities of S. verbenaca were also investigated in vitro, and phenolic compounds were determined using UHPLC-DAD-ESI-MS RESULTS: Salvia verbenaca extract contained high amounts of phenolic compounds (206 μg GAE/mg extract). The in vitro antioxidant activity showed promising radical scavenging ability, iron chelating (IC: 189 μg/mL), reducing power and strong anti-lipid-peroxidation effect (IC: 111 μg/mL). The extract had potential cytotoxic effect against Artemia salina larvae (LC: 30 μg/mL), but did not exhibit any acute/subacute toxicity effect on mice. Salvia verbenaca inhibited hypotonic and heat induced hemolysis and also reduced 50% of xylene induced ear edema at 600 mg/kg bw. Rosmarinic acid and caffeoylmalic acid were identified as the major compounds.
Salvia verbenaca hydromethanolic extract was found to be safe at acute and subacute levels. Its in vitro/in vivo antioxidant activity, membrane stabilizing properties and anti-inflammatory activity may be an important aspect of its wound healing and anti-ulcer traditional use.
已知鼠尾草属植物具有抗炎特性，传统上用于治疗目的。Salvia verbenaca 是一种阿尔及利亚植物，用于治疗伤口和溃疡。
本研究旨在评估 S. verbenaca 的急性和亚急性安全性及其作为传统应用的抗炎活性机制。
使用盐水丰年虾幼虫评估 S. verbenaca 水甲醇提取物的致死毒性，同时通过口服在小鼠中测试急性和亚急性毒性。使用膜稳定和二甲苯诱导的耳肿胀作为急性炎症模型，在体外筛选抗炎活性。还在体外研究了 S. verbenaca 的抗自由基、还原能力和铁螯合活性，并使用 UHPLC-DAD-ESI-MS 测定了酚类化合物。
鼠尾草提取物含有大量的酚类化合物（206μgGAE/mg 提取物）。体外抗氧化活性显示出有希望的自由基清除能力、铁螯合作用（IC：189μg/mL）、还原能力和强抗脂质过氧化作用（IC：111μg/mL）。该提取物对盐水丰年虾幼虫具有潜在的细胞毒性（LC：30μg/mL），但对小鼠没有表现出任何急性/亚急性毒性作用。Salvia verbenaca 抑制低渗和热诱导的溶血，并在 600mg/kg bw 时降低 50%的二甲苯诱导的耳肿胀。鉴定出迷迭香酸和咖啡酰苹果酸为主要化合物。
在急性和亚急性水平下，发现鼠尾草水甲醇提取物是安全的。其体外/体内抗氧化活性、膜稳定特性和抗炎活性可能是其伤口愈合和抗溃疡传统用途的重要方面。
