Wageningen University, Bioprocess Engineering, AlgaePARC, P.O. Box 16, 6700 AA Wageningen, Netherlands.
Wageningen University, Bioprocess Engineering, AlgaePARC, P.O. Box 16, 6700 AA Wageningen, Netherlands.
Bioresour Technol. 2021 Apr;325:124725. doi: 10.1016/j.biortech.2021.124725. Epub 2021 Jan 18.
This work aimed to select a Tisochrysis lutea phenotype with higher biomass and fucoxanthin productivities using fluorescence-activated cell sorting (FACS). A novel phenotype was obtained after 2 rounds of selection, based on high-fucoxanthin fluorescence. The resulting phenotype forms cell aggregates, has no flagella, and was stable after 15 months. Optimal temperature (30 °C) and light (300 µmol m s) were obtained at laboratory scale, identical to the original strain. The biomass productivity was higher than the original strain: 1.9× at laboratory scale (0.4 L), and 4.5× under outdoor conditions (190 L). Moreover, compared to the original strain, the productivity of fucoxanthin increased 1.6-3.1× and docosahexaenoic acid 1.5-1.9×. These are the highest ever reported outdoor productivities, obtained with a robust new phenotype from a T. lutea monoculture isolated with FACS without genetic manipulation. The resulting phenotype shows high potential for industrial production.
本研究旨在利用荧光激活细胞分选（FACS）技术筛选出具有更高生物量和岩藻黄质生产力的新月菱形藻表型。经过两轮基于高岩藻黄质荧光的筛选，获得了一种新型表型。所得表型形成细胞聚集体，无鞭毛，在 15 个月后仍保持稳定。在实验室规模下，最佳温度（30°C）和光照（300 µmol m s）与原始菌株相同。生物量生产力高于原始菌株：实验室规模下提高了 1.9 倍（0.4 L），户外条件下提高了 4.5 倍（190 L）。此外，与原始菌株相比，岩藻黄质的生产力提高了 1.6-3.1 倍，二十二碳六烯酸提高了 1.5-1.9 倍。这些是迄今为止报道的最高的户外生产力，是通过 FACS 从新月菱形藻单培养物中分离出的、未经遗传操作的稳健新型表型获得的。所得表型显示出在工业生产中的巨大潜力。
