Montpellier SupAgro, UMR 1208 IATE, 2 Place Viala, 34060 Montpellier CEDEX 2, France.
Microphyt, 713 Route de Mudaison, 34630 Baillargues, France.
Molecules. 2018 Apr 11;23(4):874. doi: 10.3390/molecules23040874.
By their autotrophic nature and their molecular richness, microalgae are serious assets in the context of current environmental and societal challenges. Some species produce both omega-3 long chain polyunsaturated fatty acids (PUFAs) and xanthophylls, two molecular families widely studied for their bioactivities in the fields of nutrition and cosmetics. Whereas most studies separately deal with the two families, synergies could be exploited with extracts containing both PUFAs and xanthophylls. The purpose of our work was to determine cost effective and eco-friendly parameters for their co-extraction. The effect of several parameters (solvent, solvent/biomass ratio, temperature, duration) were studied, using two microalgal species, the non-calcifying Haptophyta and the diatom , that presents a silicified frustule. Analyses of PUFAs and fucoxanthin (Fx), the main xanthophyll, allowed to compare kinetics and extraction yields between experimental protocols. Co-extraction yields achieved using 96% ethanol as solvent were 100% for Fx and docosahexaenoic acid (DHA) in one hour from biomass, and respectively 95% and 89% for Fx and eicosapentaenoic acid (EPA) in eight hours from . These conditions are compatible with industrial applications.
由于其自养特性和分子丰富性，微藻在当前环境和社会挑战的背景下是一项重要资源。一些物种既产生ω-3 长链多不饱和脂肪酸（PUFA）又产生叶黄素，这两种分子家族因其在营养和化妆品领域的生物活性而受到广泛研究。虽然大多数研究分别处理这两个家族，但可以利用同时含有 PUFAs 和叶黄素的提取物来发挥协同作用。我们的工作旨在确定其协同提取的经济高效且环保的参数。使用两种微藻，即非钙化的甲藻和具有硅化外壳的硅藻，研究了几种参数（溶剂、溶剂/生物量比、温度、时间）的影响。对 PUFAs 和叶黄素（Fx）（主要的叶黄素）进行分析，比较了实验方案之间的动力学和提取产率。使用 96%乙醇作为溶剂，在 1 小时内从生物量中可以实现 Fx 和二十二碳六烯酸（DHA）的协同提取产率达到 100%，在 8 小时内从 中可以实现 Fx 和二十碳五烯酸（EPA）的协同提取产率达到 95%和 89%。这些条件与工业应用兼容。
