Wageningen University, Bioprocess Engineering, AlgaePARC, P.O. Box 16, 6700 AA Wageningen, Netherlands.
Wageningen University, Bioprocess Engineering, AlgaePARC, P.O. Box 16, 6700 AA Wageningen, Netherlands.
Bioresour Technol. 2021 Feb;321:124434. doi: 10.1016/j.biortech.2020.124434. Epub 2020 Nov 20.
It is known that microalgae respond to different light colors, but not at single-cell level. This work aimed to assess if different light colors could be used as triggers to sort over-producing cells. Six light spectra were used: red + green + blue (RGBL), blue (BL), red (RL), green (GL), blue + red (BRL) and blue + green (BGL). Fluorescence-activated cell sorting method was used to analyse single-cell fluorescence and sort cells. BGL and RGBL lead to the highest fucoxanthin production, while RL showed the lowest. Therefore, it was hypothesized that hyper-producing cells can be isolated efficiently under the adverse condition (RL). After exposure to all light colors for 14 days, the top 1% fucoxanthin producing cells were sorted. A sorted strain from RL showed higher (16-19%) growth rate and fucoxanthin productivity. This study showed how light spectra affected single-cell fucoxanthin and lipid contents and productivities. Also, it supplied an approach to sort for high-fucoxanthin or high-lipid cells.
已知微藻会对不同的光色做出响应，但这种响应不是在单细胞水平上的。本工作旨在评估不同的光色是否可以作为触发因素来对高产细胞进行分选。使用了六种不同的光谱：红+绿+蓝（RGBL）、蓝（BL）、红（RL）、绿（GL）、蓝+红（BRL）和蓝+绿（BGL）。采用荧光激活细胞分选法来分析单细胞荧光并对细胞进行分选。BGL 和 RGBL 导致最高的岩藻黄质产量，而 RL 则显示出最低的产量。因此，假设在不利条件（RL）下可以有效地分离高产细胞。在暴露于所有光色 14 天后，对产岩藻黄质最多的 1%的细胞进行了分选。从 RL 中分选出来的一株菌显示出更高的（16-19%）生长速率和岩藻黄质生产力。这项研究表明了不同的光光谱如何影响单细胞岩藻黄质和脂质的含量和生产力。此外，它提供了一种分选高岩藻黄质或高脂质细胞的方法。
