利用高速逆流色谱法分离药草中的黄酮类化合物和生物碱。

Separations of flavonoids and alkaloids in medicinal herbs by high-speed counter-current chromatography.

作者信息

Zhang T Y, Cai D G, Ito Y

机构信息

Laboratory of Technical Development, National Heart, Lung, and Blood Institute, Bethesda, MD 20892.

出版信息

J Chromatogr. 1988 Jan 1;435(1):159-66. doi: 10.1016/s0021-9673(01)82171-3.

DOI:10.1016/s0021-9673(01)82171-3

PMID:3350890

Abstract

Counter-current chromatography is a new liquid-liquid partition chromatography without using solid support. Recently, the technique has been remarkably improved in both partition efficiency and separation time. In this paper the capability of this high-speed counter-current chromatography was demonstrated on separation of two sets of samples obtained from medicinal herbs: a synthetic mixture of 3'-hydroxygenkwanin, luteolin and apigenin was separated on a two-phase solvent system composed of chloroform-methanol-water (4:3:2, v/v/v) and a crude ethanol extract from Anisodus tangulicus (Maxin) Pasch on chloroform-0.07 M sodium phosphate (pH 6.4) (1:1, v/v). In the light of chromatograms obtained from these samples, advantages of high-speed counter-current chromatography over other chromatographic methods were discussed in terms of partition efficiency, peak resolution, separation time, sample loading capacity, etc.

摘要

逆流色谱是一种不使用固体载体的新型液-液分配色谱法。近年来，该技术在分配效率和分离时间方面都有了显著提高。本文展示了这种高速逆流色谱在分离两组来自草药的样品方面的能力：在由氯仿-甲醇-水（4:3:2，v/v/v）组成的两相溶剂系统上分离了3'-羟基芫花素、木犀草素和芹菜素的合成混合物，并在氯仿-0.07M磷酸钠（pH 6.4）（1:1，v/v）上分离了天仙子（Maxin）Pasch的粗乙醇提取物。根据从这些样品获得的色谱图，从分配效率、峰分辨率、分离时间、样品负载量等方面讨论了高速逆流色谱相对于其他色谱方法的优势。