高速逆流色谱法分离纯化10-脱乙酰巴卡亭III
Separation and purification of 10-deacetylbaccatin III by high-speed counter-current chromatography.
作者信息
Cao X, Tian Y, Zhang T Y, Ito Y
机构信息
Beijing Institute for New Technology Application, China.
出版信息
J Chromatogr A. 1998 Jul 17;813(2):397-401. doi: 10.1016/s0021-9673(98)00360-4.
High-speed counter-current chromatography (HSCCC) was used for the separation and purification of 10-deacetylbaccatin III from the needle extract of Chinese yew (Taxus chinensis). The crude needle extract (500 mg/5 ml) was first separated with a two-phase solvent system composed of n-hexane-ethyl acetate-ethanol-water (2:5:2:5, v/v) and the partially purified fraction was again purified with a different solvent system composed of n-hexane-chloroform-methanol-water (5:25:34:20, v/v). HPLC analysis of the final fraction showed that the purity of 10-deacetylbaccatin (20 mg) was over 98%. The chemical structure was confirmed by fast atom bombardment MS and 1H NMR.
采用高速逆流色谱法（HSCCC）从中国红豆杉（Taxus chinensis）针叶提取物中分离纯化10-去乙酰巴卡亭III。首先将粗针叶提取物（500 mg/5 ml）用正己烷 - 乙酸乙酯 - 乙醇 - 水（2:5:2:5，v/v）组成的两相溶剂系统进行分离，然后将部分纯化的馏分再用由正己烷 - 氯仿 - 甲醇 - 水（5:25:34:20，v/v）组成的不同溶剂系统进行纯化。对最终馏分的HPLC分析表明，10-去乙酰巴卡亭（20 mg）的纯度超过98%。通过快原子轰击质谱和1H NMR对化学结构进行了确证。
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