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潜伏结核感染期间 toll 样受体的体外 mRNA 表达。

Ex vivo mRNA expression of toll-like receptors during latent tuberculosis infection.

机构信息

College of Health Sciences, Department of Medical Laboratory Sciences, Woldia University, Woldia, Ethiopia.

Armauer Hansen Research Institute, Addis Ababa, Ethiopia.

出版信息

BMC Immunol. 2021 Jan 28;22(1):9. doi: 10.1186/s12865-021-00400-4.

DOI:10.1186/s12865-021-00400-4
PMID:33509080
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7842038/
Abstract

BACKGROUND

Understanding immune mechanisms, particularly the role of innate immune markers during latent TB infection remains elusive. The main objective of this study was to evaluate mRNA gene expression patterns of toll-like receptors (TLRs) as correlates of immunity during latent TB infection and further infer their roles as potential diagnostic biomarkers.

METHODS

Messenger RNA (mRNA) levels were analysed in a total of 64 samples collected from apparently healthy children and adolescents latently infected with tuberculosis (n = 32) or non-infected (n = 32). Relative expression in peripheral blood of selected genes encoding TLRs (TLR-1, TLR-2, TLR-4, TLR-6 and TLR-9) was determined with a quantitative real-time polymerase chain reaction (qRT-PCR) using specific primers and florescent labelled probes and a comparative threshold cycle method to define fold change. Data were analysed using Graph-Pad Prism 7.01 for Windows and a p-value less than 0.05 was considered statistically significant.

RESULTS

An increased mean fold change in the relative expression of TLR-2 and TLR-6 mRNA was observed in LTBI groups relative to non-LTBI groups (p < 0.05), whereas a slight fold decrease was observed for TLR-1 gene.

CONCLUSIONS

An increased mRNA expression of TLR-2 and TLR-6 was observed in latently infected individuals relative to those non-infected, possibly indicating the roles these biomarkers play in sustenance of the steady state interaction between the dormant TB bacilli and host immunity.

摘要

背景

了解免疫机制，特别是潜伏性结核病感染期间固有免疫标志物的作用，仍然难以捉摸。本研究的主要目的是评估 toll 样受体 (TLR) 的 mRNA 基因表达模式作为潜伏性结核病感染期间免疫的相关性，并进一步推断它们作为潜在诊断生物标志物的作用。

方法

从潜伏性结核病感染（n=32）或非感染（n=32）的明显健康儿童和青少年中总共收集了 64 个样本，分析了信使 RNA (mRNA) 水平。使用定量实时聚合酶链反应 (qRT-PCR) 用特异性引物和荧光标记探针以及比较阈值循环方法来确定编码 TLRs（TLR-1、TLR-2、TLR-4、TLR-6 和 TLR-9）的选定基因在外周血中的相对表达，并定义倍数变化。使用 Graph-Pad Prism 7.01 for Windows 分析数据，p 值小于 0.05 被认为具有统计学意义。

结果

潜伏性结核病感染组 TLR-2 和 TLR-6 mRNA 的相对表达平均倍数变化增加，与非潜伏性结核病感染组相比（p<0.05），而 TLR-1 基因的倍数变化略有下降。

结论

潜伏性感染个体中 TLR-2 和 TLR-6 的 mRNA 表达增加，可能表明这些生物标志物在维持休眠结核杆菌与宿主免疫之间的稳态相互作用中发挥作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf1d/7842038/45540fc225ce/12865_2021_400_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf1d/7842038/c96e0f3a6aa0/12865_2021_400_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf1d/7842038/3643dc201c60/12865_2021_400_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf1d/7842038/d91be3dd0571/12865_2021_400_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf1d/7842038/67ed421c57ba/12865_2021_400_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf1d/7842038/45540fc225ce/12865_2021_400_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf1d/7842038/c96e0f3a6aa0/12865_2021_400_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf1d/7842038/3643dc201c60/12865_2021_400_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf1d/7842038/d91be3dd0571/12865_2021_400_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf1d/7842038/67ed421c57ba/12865_2021_400_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf1d/7842038/45540fc225ce/12865_2021_400_Fig5_HTML.jpg

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