Yaginuma T, Happ G M
Department of Zoology, University of Vermont, Burlington 05405.
J Comp Physiol B. 1988;157(6):765-70. doi: 10.1007/BF00691007.
In Tenebrio molitor, male adults transfer sperm to the female via a spermatophore or sperm sac. The spermatophore is formed from secretions of the bean-shaped accessory glands (BAGs) and the tubular accessory glands (TAGs) of the male beetle. Trehalase is found in the adult BAGs. During the pupal stage, the activity in the BAGs was very low. After adult ecdysis, the total activity increased 100-fold from 0 days to 6 days and reached maximum levels at 9 days. The specific activity increased 20-fold from the time of ecdysis to 6 days thereafter. In the 10 day adult, trehalase levels in testes, seminal vesicles, vas deferens, TAGs, or ejaculatory ducts, were lower by two orders of magnitude than in the BAGs. However, the specific activity in the spermatophore was similar to that in the BAGs. Trehalases in the BAGs and the spermatophores showed very similar properties (soluble, optimum pH of 5.75 and Km value of 5.4 mM for trehalose). Thus trehalase appears to be secreted from the BAGs and becomes incorporated into the spermatophores.
在黄粉虫中，成年雄性通过精荚或精囊将精子传递给雌性。精荚由雄性甲虫的豆形附腺（BAGs）和管状附腺（TAGs）的分泌物形成。海藻糖酶存在于成年BAGs中。在蛹期，BAGs中的活性非常低。成虫蜕皮后，总活性从0天到6天增加了100倍，并在9天达到最高水平。比活性从蜕皮时到此后6天增加了20倍。在10日龄成虫中，睾丸、精囊、输精管、TAGs或射精管中的海藻糖酶水平比BAGs中的低两个数量级。然而，精荚中的比活性与BAGs中的相似。BAGs和精荚中的海藻糖酶表现出非常相似的特性（可溶，海藻糖的最适pH为5.75，Km值为5.4 mM）。因此，海藻糖酶似乎是从BAGs分泌出来并整合到精荚中的。
