Takiguchi M, Niimi T, Su Z H, Yaginuma T
Laboratory of Sericultural Science, Faculty of Agriculture, Nagoya University, Japan.
Biochem J. 1992 Nov 15;288 ( Pt 1)(Pt 1):19-22. doi: 10.1042/bj2880019.
A cDNA of alpha alpha-trehalase (EC 3.2.1.28) from a cDNA library of male bean-shaped accessory gland of the mealworm beetle, Tenebrio molitor, has been isolated by the homology screening approach. Sequence analysis of the cDNA (1830 bp) revealed that the cDNA encoded a protein of 555 amino acids with a calculated M(r) of 64457. The deduced amino acid sequence had significant similarities to rabbit small intestine and Escherichia coli trehalases. Northern blotting and semi-quantitative PCR analyses revealed that a trehalase transcript with about 2.0 kb was abundant in bean-shaped accessory glands. In the glands, the amount of trehalase transcript increased from 1 to 2 days after adult ecdysis. These tissue- and stage-specific gene expressions of trehalase corresponded to the tissue- and stage-specificity of trehalase activity.
通过同源性筛选方法，从黄粉虫（Tenebrio molitor）雄性豆形附腺的cDNA文库中分离出αα - 海藻糖酶（EC 3.2.1.28）的cDNA。对该cDNA（1830 bp）的序列分析表明，该cDNA编码一个由555个氨基酸组成的蛋白质，计算所得的分子量（M(r)）为64457。推导的氨基酸序列与兔小肠和大肠杆菌海藻糖酶具有显著相似性。Northern印迹和半定量PCR分析显示，在豆形附腺中，约2.0 kb的海藻糖酶转录本丰富。在这些腺体中，海藻糖酶转录本的量在成虫蜕皮后1至2天增加。海藻糖酶的这些组织和阶段特异性基因表达与海藻糖酶活性的组织和阶段特异性相对应。
