Feng X, Happ G M
Department of Biology, University of Vermont, Burlington 05405-0086, USA.
Gene. 1996 Nov 14;179(2):257-62. doi: 10.1016/s0378-1119(96)00372-1.
The cDNA for Sp23, a structural protein of the spermatophore of Tenebrio molitor, had been previously cloned and characterized (Paesen, G.C., Schwartz, M.B., Peferoen, M., Weyda, F. and Happ, G.M. (1992a) Amino acid sequence of Sp23, a structure protein of the spermatophore of the mealworm beetle, Tenebrio molitor. J. Biol. Chem. 257, 18852-18857). Using the labeled cDNA for Sp23 as a probe to screen a library of genomic DNA from Tenebrio molitor, we isolated a genomic clone for Sp23. A 5373-base pair (bp) restriction fragment containing the Sp23 gene was sequenced. The coding region is separated by a 55-bp intron which is located close to the translation start site. Three putative ecdysone response elements (EcRE) are identified in the 5' flanking region of the Sp23 gene. Comparison of the flanking regions of the Sp23 gene with those of the D-protein gene expressed in the accessory glands of Tenebrio reveals similar sequences present in the flanking regions of the two genes. The genomic organization of the coding region of the Sp23 gene shares similarities with that of the D-protein gene, three Drosophila accessory gland genes and two Drosophila 20-OH ecdysone-responsive genes.
黄粉虫精子包囊结构蛋白Sp23的cDNA此前已被克隆并进行了表征（帕森斯，G.C.，施瓦茨，M.B.，佩费伦，M.，韦达，F.和哈普，G.M.（1992a）黄粉虫精子包囊结构蛋白Sp23的氨基酸序列。《生物化学杂志》257，18852 - 18857）。利用标记的Sp23 cDNA作为探针筛选黄粉虫基因组DNA文库，我们分离出了Sp23的基因组克隆。对一个包含Sp23基因的5373碱基对（bp）的限制性片段进行了测序。编码区被一个位于靠近翻译起始位点的55 bp内含子隔开。在Sp23基因的5'侧翼区域鉴定出三个假定的蜕皮激素反应元件（EcRE）。将Sp23基因的侧翼区域与黄粉虫附属腺中表达的D蛋白基因的侧翼区域进行比较，发现这两个基因的侧翼区域存在相似序列。Sp23基因编码区的基因组组织与D蛋白基因、三个果蝇附属腺基因和两个果蝇20 - OH蜕皮激素反应基因的基因组组织相似。
