International Rice Research Institute (IRRI), DAPO Box 7777, Metro Manila, Philippines.
Philippines Rice Research Institute (PhilRice), Maligaya, Science City of Munoz, Philippines.
Sci Rep. 2021 Jan 28;11(1):2496. doi: 10.1038/s41598-021-82001-0.
Golden Rice with β-carotene in the grain helps to address the problem of vitamin A deficiency. Prior to commercialize Golden Rice, several performance and regulatory checkpoints must be achieved. We report results of marker assisted backcross breeding of the GR2E trait into three popular rice varieties followed by a series of confined field tests of event GR2E introgression lines to assess their agronomic performance and carotenoid expression. Results from confined tests in the Philippines and Bangladesh have shown that GR2E introgression lines matched the performance of the recurrent parents for agronomic and yield performance, and the key components of grain quality. Moreover, no differences were observed in terms of pest and disease reaction. The best performing lines identified in each genetic background had significant amounts of carotenoids in the milled grains. These lines can supply 30-50% of the estimated average requirements of vitamin A.
富含β-胡萝卜素的“金色大米”有助于解决维生素 A 缺乏症问题。在将“金色大米”商业化之前，必须达到几个性能和监管的检查点。我们报告了将 GR2E 性状标记辅助回交到三种流行的水稻品种中的结果，随后对事件 GR2E 导入系进行了一系列封闭田间试验，以评估它们的农艺性能和类胡萝卜素表达。在菲律宾和孟加拉国进行的封闭试验结果表明，GR2E 导入系在农艺和产量性能以及谷物质量的关键成分方面与轮回亲本的表现相匹配。此外，在病虫害反应方面没有观察到差异。在每个遗传背景中表现最好的鉴定线在碾磨的谷物中有大量的类胡萝卜素。这些线可以提供估计平均维生素 A 需要量的 30-50%。
