Sun Meilin, Han Kai, Hu Rui, Liu Dan, Fu Wenzhu, Liu Wenming
School of Basic Medical Science, Central South University, Changsha, Hunan, 410013, China.
Adv Healthc Mater. 2021 Apr;10(7):e2001545. doi: 10.1002/adhm.202001545. Epub 2021 Jan 29.
Porous membrane materials at the micro/nanoscale have exhibited practical and potential value for extensive biological and medical applications associated with filtration and isolation, cell separation and sorting, micro-arrangement, in-vitro tissue reconstruction, high-throughput manipulation and analysis, and real-time sensing. Herein, an overview of technological development of micro/nanoporous membranes (M/N-PMs) is provided. Various membrane types and the progress documented in membrane fabrication techniques, including the electrochemical-etching, laser-based technology, microcontact printing, electron beam lithography, imprinting, capillary force lithography, spin coating, and microfluidic molding are described. Their key features, achievements, and limitations associated with micro/nanoporous membrane (M/N-PM) preparation are discussed. The recently popularized applications of M/N-PMs in biomedical engineering involving the separation of cells and biomolecules, bioparticle operations, biomimicking, micropatterning, bioassay, and biosensing are explored too. Finally, the challenges that need to be overcome for M/N-PM fabrication and future applications are highlighted.
微纳尺度的多孔膜材料在与过滤和分离、细胞分离与分选、微排列、体外组织重建、高通量操作与分析以及实时传感相关的广泛生物和医学应用中展现出了实际和潜在的价值。在此，对微纳多孔膜（M/N-PMs）的技术发展进行概述。描述了各种膜类型以及膜制备技术方面的进展，包括电化学蚀刻、激光技术、微接触印刷、电子束光刻、压印、毛细力光刻、旋涂和微流控成型。讨论了与微纳多孔膜（M/N-PM）制备相关的关键特性、成果和局限性。还探讨了M/N-PMs最近在生物医学工程中的广泛应用，包括细胞和生物分子的分离、生物颗粒操作、仿生、微图案化、生物测定和生物传感。最后，强调了M/N-PM制造和未来应用需要克服的挑战。
