基于红细胞膜功能化等离子体纳米颗粒的凝血启发式直接纤维蛋白原检测法
Coagulation-Inspired Direct Fibrinogen Assay Using Plasmonic Nanoparticles Functionalized with Red Blood Cell Membranes.
作者信息
Kim Insu, Lee Dongtak, Lee Sang Won, Lee Jeong Hoon, Lee Gyudo, Yoon Dae Sung
机构信息
School of Biomedical Engineering, Korea University, Seoul 02841, Republic of Korea.
Department of Electrical Engineering, Kwangwoon University, Seoul 01897, Republic of Korea.
出版信息
ACS Nano. 2021 Apr 27;15(4):6386-6394. doi: 10.1021/acsnano.0c08136. Epub 2021 Jan 29.
The fast measurement of fibrinogen is essential in evaluating life-threatening sepsis and cardiovascular diseases. Here, we aim to utilize biomimetic plasmonic Au nanoparticles using red blood cell membranes (RBCM-AuNPs) and demonstrate nanoscale coagulation-inspired fibrinogen detection cross-linking between RBCM-AuNPs. The proposed biomimetic RBCM-AuNPs are highly suitable for fibrinogen detection because hemagglutination, occurring in the presence of fibrinogen, induces a shift in the localized surface plasmon resonance of the NPs. Specifically, when the two ends of the fibrinogen protein are bound to receptors on separate RBCM-AuNPs, cross-linking of the RBCM-AuNPs occurs, yielding a corresponding plasmon shift within 10 min. This coagulation-inspired fibrinogen detection method, with a low sample volume, high selectivity, and high speed, could facilitate the diagnosis of sepsis and cardiovascular diseases.
快速检测纤维蛋白原对于评估危及生命的败血症和心血管疾病至关重要。在此，我们旨在利用红细胞膜仿生等离子体金纳米颗粒（RBCM-AuNPs），并展示受纳米级凝血启发的RBCM-AuNPs之间的纤维蛋白原检测交联。所提出的仿生RBCM-AuNPs非常适合用于纤维蛋白原检测，因为在纤维蛋白原存在下发生的血凝反应会引起纳米颗粒局部表面等离子体共振的偏移。具体而言，当纤维蛋白原蛋白的两端与不同RBCM-AuNPs上的受体结合时，RBCM-AuNPs会发生交联，在10分钟内产生相应的等离子体偏移。这种受凝血启发的纤维蛋白原检测方法，具有低样本量、高选择性和高速度，有助于败血症和心血管疾病的诊断。
Zotero 插件