Department of Fundamental, Prophylactic and Clinical Disciplines, Faculty of Medicine, Transilvania University of Brasov, Brașov, Romania.
Research Center for Fundamental Research and Prevention Strategies in Medicine, Research and Development Institute of Transilvania University of Brasov, Brașov, Romania.
Anal Bioanal Chem. 2024 Jan;416(1):21-36. doi: 10.1007/s00216-023-04976-1. Epub 2023 Oct 14.
Fibrinogen as a major inflammation marker and blood coagulation factor has a direct impact on the health of humanity. The variations in fibrinogen content lead to risky conditions such as bleeding and cardiovascular diseases. So, accurate methods for monitoring of this glycoprotein are of high importance. The conventional methods, such as the Clauss method, are time consuming and require highly specialized expert analysts. The development of fast, simple, easy to use, and inexpensive methods is highly desired. In this way, biosensors have gained outstanding attention since they offer means for performing analyses at the points-of-care using self-testing devices, which can be applied outside of clinical laboratories or hospital. This review indicates that different electrochemical and optical sensors have been successfully implemented for the detection of fibrinogen under normal levels of fibrinogen in plasma. The biosensors for the detection of fibrinogen have been designed based on the quartz crystal microbalance, field-effect transistor, electrochemical impedance spectroscopy, amperometry, surface plasmon resonance, localized surface plasmon resonance, and colorimetric techniques. Also, this review demonstrates the utility of the application of nanoparticles in different detection techniques.
纤维蛋白原为主要炎症标志物和凝血因子，直接影响人类健康。纤维蛋白原含量的变化会导致出血和心血管疾病等危险情况。因此，监测这种糖蛋白的准确方法非常重要。传统方法，如 Clauss 法，耗时且需要高度专业化的专家分析。因此，人们非常希望开发快速、简单、易于使用且廉价的方法。在这种情况下，生物传感器因其提供了使用自检设备在护理点进行分析的手段而引起了极大的关注，这些设备可在临床实验室或医院外使用。本文综述表明，已经成功地实现了不同的电化学和光学传感器来检测血浆中正常纤维蛋白原水平下的纤维蛋白原。基于石英晶体微天平、场效应晶体管、电化学阻抗谱、安培法、表面等离子体共振、局域表面等离子体共振和比色技术设计了用于检测纤维蛋白原的生物传感器。此外，本文综述还展示了纳米粒子在不同检测技术中的应用的实用性。
