Department of Nutrition and Food Hygiene, College of Public Health, Xinjiang Medical University, Urumqi, Xinjiang, China.
J Med Food. 2021 Feb;24(2):124-134. doi: 10.1089/jmf.2020.4800. Epub 2021 Jan 28.
Hyperlipidemia is a metabolic disorder characterized by high lipid levels, which may lead to cardiovascular diseases. Evidence suggests that improving the gut microbiota homeostasis is of great importance in lipid metabolism. Dietary fiber may positively regulate blood lipid and intestinal microbiota, therefore, we have investigated the effect of chickpea dietary fiber (CDF) on high-fat diet (HFD)-induced hyperlipidemia and gut bacterial dysbiosis. Fifty male Sprague Dawley rats purchased for this study were randomly divided into 5 groups of 10 rats each. The control group was fed with normal diet (ND), while the other four groups were all fed with HFD for inducing hyperlipidemia. Then one of the four HFD groups continued to be fed with only HFD, and the other three groups were fed with CDF in different doses: high CDF (30 g CDF/kg of HFD), medium CDF (15 g CDF/kg of HFD), and low CDF (5 g CDF/kg of HFD). After CDF treatment, the lipid level in serum was determined through biochemical methods, and microbial content of the fecal sample was determined by 16S rDNA sequencing. We found that CDF could decrease the levels of total cholesterol, triglyceride, and low-density lipoprotein cholesterol and increase the level of high-density lipoprotein cholesterol significantly. The diversity of gut microbiota in the ND group and CDF-treated groups were higher than HFD group. The -diversity analysis showed that there were significant differences in gut microbiota among HFD-, ND-, and CDF-treated groups. Rats in CDF groups tended to be similar and interactive. CDF can effectively increase the abundance of and in rats and increase the level of propionic acid. These results indicated that CDF might affect serum lipid and gut bacterial ecosystem positively.
高脂血症是一种代谢紊乱，其特征是血脂水平升高，可能导致心血管疾病。有证据表明，改善肠道微生物组稳态对脂质代谢非常重要。膳食纤维可能对血脂和肠道微生物群有积极的调节作用，因此，我们研究了鹰嘴豆膳食纤维（CDF）对高脂肪饮食（HFD）诱导的高脂血症和肠道细菌失调的影响。这项研究购买了 50 只雄性 Sprague Dawley 大鼠，随机分为 5 组，每组 10 只。对照组喂食正常饮食（ND），其余 4 组均喂食 HFD 以诱导高脂血症。然后，其中一组继续只喂食 HFD，另外三组喂食不同剂量的 CDF：高 CDF（30g CDF/kg HFD）、中 CDF（15g CDF/kg HFD）和低 CDF（5g CDF/kg HFD）。在 CDF 处理后，通过生化方法测定血清中的脂质水平，并通过 16S rDNA 测序测定粪便样本中的微生物含量。结果发现，CDF 可显著降低总胆固醇、甘油三酯和低密度脂蛋白胆固醇水平，提高高密度脂蛋白胆固醇水平。ND 组和 CDF 处理组的肠道微生物多样性高于 HFD 组。-多样性分析表明，HFD、ND 和 CDF 处理组之间的肠道微生物群存在显著差异。CDF 组的大鼠趋于相似和相互作用。CDF 可有效增加大鼠中的 和 的丰度，并增加丙酸水平。这些结果表明，CDF 可能对血清脂质和肠道细菌生态系统产生积极影响。
