Shanghai Public Health Clinical Center, Shanghai Medical College, Fudan University, Shanghai 201508, China.
Shanghai Public Health Clinical Center, Shanghai Medical College, Fudan University, Shanghai 201508, China; Department of Medical Biotechnology, Yeungnam University, Gyeongsan 38541, South Korea; Research Institute of Cell Culture, Yeungnam University, Gyeongsan 38541, South Korea.
Int J Biol Macromol. 2021 Mar 31;174:477-484. doi: 10.1016/j.ijbiomac.2021.01.155. Epub 2021 Jan 26.
Brown seaweed is an important source of fucoidan, which displays immunomodulatory effects by activating various immune cells. However, these effects of fucoidans from various sources of brown seaweed have not yet been explored in human blood dendritic cells. We studied fucoidans extracted from Ecklonia cava, Macrocystis pyrifera, Undaria pinnatifida, and Fucus vesiculosus for their effects on human monocyte-derived dendritic cells (MODC) and human peripheral blood DC (PBDC) activation. Ecklonia cava fucoidan (ECF) strongly upregulated co-stimulatory molecules, major histocompatibility complex class I and II, and the production of proinflammatory cytokines in MODCs and PBDCs compared to those by the other three fucoidans. Moreover, ECF elicited the strongest effect in the induction of syngeneic T cell proliferation and IFN-γ production compared to those of other fucoidans. These results suggest that ECF could be a suitable candidate molecule for enhancing immune activation in humans compared to that with the other three fucoidans.
褐藻是岩藻聚糖硫酸酯的重要来源，它通过激活各种免疫细胞来发挥免疫调节作用。然而，这些来自不同褐藻来源的岩藻聚糖硫酸酯在人类血液树突状细胞中的作用尚未得到探索。我们研究了从裙带菜、巨藻、昆布和泡叶藻中提取的岩藻聚糖硫酸酯对人单核细胞来源的树突状细胞（MODC）和人外周血树突状细胞（PBDC）激活的影响。与其他三种岩藻聚糖硫酸酯相比，裙带菜岩藻聚糖硫酸酯（ECF）能强烈上调共刺激分子、主要组织相容性复合体 I 类和 II 类，并促进 MODC 和 PBDC 中前炎性细胞因子的产生。此外，与其他三种岩藻聚糖硫酸酯相比，ECF 能在诱导同种异体 T 细胞增殖和 IFN-γ产生方面产生最强的作用。这些结果表明，与其他三种岩藻聚糖硫酸酯相比，ECF 可能是一种增强人类免疫激活的合适候选分子。
