School of Health Sciences, University of Tasmania, Launceston, TAS 7248, Australia.
Respiratory Translational Research Group, Department of Laboratory Medicine, School of Health Sciences, College of Health and Medicine, University of Tasmania, Launceston, TAS 7248, Australia.
Mar Drugs. 2021 Dec 11;19(12):702. doi: 10.3390/md19120702.
Fucoidans are sulfated, complex, fucose-rich polymers found in brown seaweeds. Fucoidans have been shown to have multiple bioactivities, including anti-inflammatory effects, and are known to inhibit inflammatory processes via a number of pathways such as selectin blockade and enzyme inhibition, and have demonstrated inhibition of inflammatory pathologies . In this current investigation, fucoidan extracts from and were assessed for modulation of pro-inflammatory cytokine production (TNF-α, IL-1β, and IL-6) by human peripheral blood mononuclear cells (PBMCs) and in a human macrophage line (THP-1). Fucoidan extracts exhibited no signs of cytotoxicity in THP-1 cells after incubation of 48 h. Additionally, all fucoidan extracts reduced cytokine production in LPS stimulated PBMCs and human THP-1 cells in a dose-dependent fashion. Notably, the 5-30 kDa subfraction from was a highly effective inhibitor at lower concentrations. Fucoidan extracts from all species had significant anti-inflammatory effects, but the lowest molecular weight subfractions had maximal effects at low concentrations. These observations on various fucoidan extracts offer insight into strategies that improve their efficacy against inflammation-related pathology. Further studies should be conducted to elucidate the mechanism of action of these extracts.
褐藻中的岩藻聚糖硫酸酯是一种复杂的富含岩藻糖的多糖。已证实岩藻聚糖具有多种生物活性，包括抗炎作用，并且已知通过多种途径（如选择素阻断和酶抑制）抑制炎症过程，并已证明抑制炎症病理学。在本研究中，评估了 和 的岩藻聚糖提取物对人外周血单核细胞（PBMCs）和人巨噬细胞系（THP-1）中促炎细胞因子（TNF-α、IL-1β 和 IL-6）产生的调节作用。岩藻聚糖提取物在孵育 48 小时后在 THP-1 细胞中没有显示出细胞毒性的迹象。此外，所有岩藻聚糖提取物均以剂量依赖性方式减少 LPS 刺激的 PBMC 和人 THP-1 细胞中的细胞因子产生。值得注意的是，来自 的 5-30 kDa 亚组分在较低浓度下是一种非常有效的抑制剂。所有物种的岩藻聚糖提取物均具有显著的抗炎作用，但在低浓度下，最低分子量的亚组分具有最大的作用。这些关于各种岩藻聚糖提取物的观察结果为提高其对抗炎症相关病理学的功效的策略提供了思路。应进行进一步的研究以阐明这些提取物的作用机制。
