Yu Yun-Sang, Jin Soyeong, Cho Namjoon, Lim Jongok, Kim Cheol-Hak, Lee Seung-Gyu, Kim Sangil, Park Jong-Seok, Kim Keekwang, Park Chungoo, Cho Sung-Jin
School of Biological Sciences, College of Natural Sciences, Chungbuk National University, Cheongju 28644, Korea.
School of Biological Sciences and Technology, Chonnam National University, Gwangju 61186, Korea.
Insects. 2021 Jan 27;12(2):111. doi: 10.3390/insects12020111.
We estimated the genome size of a relict longhorn beetle, Semenov (Cerambycidae: Prioninae)-the Korean natural monument no. 218 and a Class I endangered species-using a combination of flow cytometry and -mer analysis. The two independent methods enabled accurate estimation of the genome size in Cerambycidae for the first time. The genome size of was 1.8 ± 0.2 Gb, representing one of the largest cerambycid genomes studied to date. An accurate estimation of genome size of a critically endangered longhorned beetle is a major milestone in our understanding and characterization of the genome. Ultimately, the findings provide useful insight into insect genomics and genome size evolution, particularly among beetles.
我们使用流式细胞术和k-mer分析相结合的方法，估算了一种残遗天牛——谢米诺夫天牛（鞘翅目：锯天牛亚科）的基因组大小。谢米诺夫天牛是韩国第218号天然纪念物，也是一级濒危物种。这两种独立的方法首次实现了对天牛科昆虫基因组大小的准确估算。谢米诺夫天牛的基因组大小为1.8±0.2Gb，是迄今为止所研究的最大的天牛基因组之一。准确估算一种极度濒危天牛的基因组大小，是我们在了解和描述该基因组方面的一个重要里程碑。最终，这些发现为昆虫基因组学和基因组大小进化，尤其是甲虫的基因组学和基因组大小进化，提供了有益的见解。
