Rice G E
Department of Physiology, Monash University, Clayton, Victoria, Australia.
J Endocrinol. 1988 Feb;116(2):267-72. doi: 10.1677/joe.0.1160267.
Luteal oxytocin-containing secretory granules have been isolated and characterized in terms of their physicochemical parameters. The isopynic sedimentation density (1.03 +/- 0.003 g/ml) and sedimentation coefficient (1670 S, 0.32 mol sucrose/l, 4 degrees C) of these granules have been estimated. Based upon these estimates, the average vesicle diameter (258 +/- 17 nm) and vesicle weight (9.92 +/- 0.67 fg/vesicle) were calculated. The exchangeable water content (58.2%) of these granules was determined using density gradients prepared with deuterium oxide. Luteal oxytocin-containing granules displayed similar physicochemical characteristics to those reported for neurohypophysial peptide-containing granules, with the exception of particle size. Luteal granules were 1.3 times greater in diameter than neurohypophysial granules.
含黄体催产素的分泌颗粒已被分离出来，并根据其物理化学参数进行了表征。已估算出这些颗粒的等密度沉降密度（1.03±0.003克/毫升）和沉降系数（1670 S，0.32摩尔蔗糖/升，4℃）。基于这些估算值，计算出了平均囊泡直径（258±17纳米）和囊泡重量（9.92±0.67飞克/囊泡）。使用氧化氘制备的密度梯度测定了这些颗粒的可交换水含量（58.2%）。含黄体催产素的颗粒显示出与报道的含神经垂体肽颗粒相似的物理化学特征，但颗粒大小除外。黄体颗粒的直径比神经垂体颗粒大1.3倍。
