Gemmell R T, Quirk S J, Jenkin G, Thorburn G D
Cell Tissue Res. 1983;230(3):631-8. doi: 10.1007/BF00216206.
The granulosa luteal cells of the sheep corpus luteum secrete their hormonal products by exocytosis of granules. Electron micrographs of randomly selected granulosa cells from nine corpora lutea at day 10 of the oestrous cycle were examined to obtain the cellular density of these granules. From the area of the cell, calculated using an x-y digitiser, and the number of granules observed, the number of intracellular secretory granules per microns 3 of luteal cell cytoplasm was calculated. There was a large variation in the number of granules per cell within the same corpus luteum and between corpora lutea taken at the same stage of the cycle. The number of intracellular granules in nine corpora lutea varied from 2.12 +/- 1.05 granules per microns 3 (mean, SD, n = 30) cells to 0.36 +/- 0.18 granules per microns 3 (mean, SD, n = 26). These morphological data suggest that the variation in granule synthesis in individual cells may contribute to the variation in hormone content of corpora lutea at the same stage of the cycle, and the episodic release of hormone into the plasma.
绵羊黄体的颗粒黄体细胞通过颗粒的胞吐作用分泌其激素产物。对发情周期第10天从9个黄体中随机选取的颗粒细胞进行电子显微镜检查，以获得这些颗粒的细胞密度。根据使用xy数字化仪计算出的细胞面积以及观察到的颗粒数量，计算出每立方微米黄体细胞胞质内的细胞内分泌颗粒数量。在同一黄体内部以及处于周期同一阶段的不同黄体之间，每个细胞的颗粒数量存在很大差异。9个黄体中细胞内颗粒的数量从每立方微米2.12±1.05个颗粒（平均值、标准差，n = 30）细胞到每立方微米0.36±0.18个颗粒（平均值、标准差，n = 26）不等。这些形态学数据表明，单个细胞中颗粒合成的变化可能导致同一周期阶段黄体激素含量的变化以及激素向血浆中的间歇性释放。
