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长链非编码 RNA WT1-AS 通过下调 lncRNA UCA1 抑制非小细胞肺癌并预测不良预后。

LncRNA WT1-AS downregulates lncRNA UCA1 to suppress non-small cell lung cancer and predicts poor survival.

机构信息

Department of Thoracic Surgery, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, No.3002, Sungang west road, Futian district, Shenzhen City, 518000, People's Republic of China.

出版信息

BMC Cancer. 2021 Jan 29;21(1):104. doi: 10.1186/s12885-020-07767-4.

DOI:10.1186/s12885-020-07767-4
PMID:33514344
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7844960/
Abstract

BACKGROUND

LncRNA WT1-AS inhibits gastric cancer, while its role in other cancers is unknown. We investigated the role of WT1-AS in non-small cell lung cancer (NSCLC).

METHODS

Sixty-six NSCLC patients (40 males and 26 females; 36 to 68 years old; mean age 52.7 ± 6.4 years old) were selected from the 178 NSCLC patients operated on for lung cancer between 2010 and 2013. RT-qPCR was used to analyze the expression of lncRNA. Overexpression experiments were performed to assess interactions between lncRNAs. CCK-8 assay was carried to evaluate the roles of WT1-AS and UCA1 in regulating cell proliferation. Cell invasion and migration assays were performed to assess the roles of WT1-AS and UCA1 in regulating cell invasion and migration. Western-blot was performed to illustrate the effect of WT1-AS and UCA1 in EMT.

RESULTS

WT1-AS was downregulated in NSCLC and was correlated with poor survival. The expression of WT1-AS in NSCLC was not correlated with clinical stages. LncRNA UCA1 was upregulated in cancer tissues and inversely correlated with WT1-AS. Overexpression of UCA1 did not affect WT1-AS, while overexpression of WT1-AS led to inhibited expression of UCA1. Overexpression of UCA1 resulted in increased proliferation, EMT, migration and invasion of NSCLC cells, while overexpression of WT1-AS showed opposite effects. In addition, overexpression of UCA1 inhibited the role of overexpression of WT1-AS.

CONCLUSIONS

Therefore, overexpression of WT1-AS may inhibit the cell proliferation and EMT to decrease cell migration and invasion of NSCLC cells by downregulating UCA1.

摘要

背景

LncRNA WT1-AS 抑制胃癌，但其在其他癌症中的作用尚不清楚。我们研究了 WT1-AS 在非小细胞肺癌（NSCLC）中的作用。

方法

从 2010 年至 2013 年期间因肺癌接受手术的 178 例 NSCLC 患者中选择 66 例 NSCLC 患者（40 名男性和 26 名女性；36 至 68 岁；平均年龄 52.7 ± 6.4 岁）。采用 RT-qPCR 分析长链非编码 RNA 的表达。进行过表达实验以评估 lncRNA 之间的相互作用。CCK-8 检测用于评估 WT1-AS 和 UCA1 调节细胞增殖的作用。细胞侵袭和迁移实验用于评估 WT1-AS 和 UCA1 调节细胞侵袭和迁移的作用。Western-blot 用于说明 WT1-AS 和 UCA1 在 EMT 中的作用。

结果

WT1-AS 在 NSCLC 中下调，并与不良预后相关。WT1-AS 在 NSCLC 中的表达与临床分期无关。在癌组织中上调了 lncRNA UCA1，与 WT1-AS 呈负相关。过表达 UCA1 不会影响 WT1-AS，而过表达 WT1-AS 导致 UCA1 的表达受到抑制。过表达 UCA1 导致 NSCLC 细胞增殖、EMT、迁移和侵袭增加，而过表达 WT1-AS 则显示相反的作用。此外，过表达 UCA1 抑制了过表达 WT1-AS 的作用。

结论

因此，过表达 WT1-AS 可能通过下调 UCA1 抑制 NSCLC 细胞的增殖和 EMT，从而减少细胞迁移和侵袭。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f03/7844960/46446b47b9cb/12885_2020_7767_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f03/7844960/64c4cc4d2104/12885_2020_7767_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f03/7844960/459993476e3d/12885_2020_7767_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f03/7844960/29513e7543e7/12885_2020_7767_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f03/7844960/38bf1ccbee1e/12885_2020_7767_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f03/7844960/c54d9aa2ddff/12885_2020_7767_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f03/7844960/46446b47b9cb/12885_2020_7767_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f03/7844960/64c4cc4d2104/12885_2020_7767_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f03/7844960/459993476e3d/12885_2020_7767_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f03/7844960/29513e7543e7/12885_2020_7767_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f03/7844960/38bf1ccbee1e/12885_2020_7767_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f03/7844960/c54d9aa2ddff/12885_2020_7767_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f03/7844960/46446b47b9cb/12885_2020_7767_Fig6_HTML.jpg

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