长链非编码 RNA WTAPP1 通过下调 lncRNA HAND2-AS1 促进 NSCLC 中的癌细胞侵袭和迁移。
LncRNA WTAPP1 promotes cancer cell invasion and migration in NSCLC by downregulating lncRNA HAND2-AS1.
机构信息
Department of Anesthesiology, The Second Hospital of Jilin University, Changchun City, Jilin Province, 130041, People's Republic of China.
Department of Thoracic Surgery, The Second Hospital of Jilin University, No.218 Ziqiang Street, Changchun City, Jilin Province, 130041, People's Republic of China.
出版信息
BMC Pulm Med. 2020 May 30;20(1):153. doi: 10.1186/s12890-020-01180-0.
BACKGROUND
Long non-coding RNA (lncRNA) Wilms Tumor 1 Associated Protein Pseudogene 1 (WTAPP1) has been reported to be a critical player in the angiogenesis and migration of endothelial progenitor cells, while its involvement in cancer biology remains unknown. This study was carried out to investigate the role of WTAPP1 in non-small cell lung cancer (NSCLC).
METHODS
The expression of WTAPP1 and lncRNA HAND2 Antisense RNA 1 (HAND2-AS1) in plasma and tissues from NSCLC patients was detected by qRT-PCR. A 5-year follow-up study was carried out to explore the prognostic value of WTAPP1 for NSCLC. Overexpression experiments were performed to analyze the interaction between WTAPP1 and HAND2-AS1. Cell invasion and migration were evaluated by Transwell assays.
RESULTS
The expression of WTAPP1 was upregulated in NSCLC. The survival analysis showed that low plasma levels of WTAPP1 were accompanied with high survival rate. HAND2-AS1 was downregulated in NSCLC and inversely correlated with WTAPP1 across tumor tissues. Overexpression of WTAPP1 resulted in downregulation of HAND2-AS1 in NSCLC cells, while overexpression of HAND2-AS1 did not affect the expression of WTAPP1. Overexpression of WTAPP1 led to promoted, while overexpression of HAND2-AS1 resulted in inhibited invasion and migration of NSCLC cells. In addition, overexpression of HAND2-AS1 partially attenuated the effects of overexpressing WTAPP1. In addition, WTAPP1 did not affect cancer cell proliferation.
CONCLUSION
WTAPP1 may promote cancer cell invasion and migration in NSCLC by downregulating lncRNA HAND2-AS1.
背景
长链非编码 RNA（lncRNA）Wilms 肿瘤 1 相关蛋白假基因 1（WTAPP1）已被报道在血管生成和内皮祖细胞迁移中发挥关键作用，而其在癌症生物学中的作用尚不清楚。本研究旨在探讨 WTAPP1 在非小细胞肺癌（NSCLC）中的作用。
方法
通过 qRT-PCR 检测 NSCLC 患者血浆和组织中 WTAPP1 和 lncRNA HAND2 反义 RNA 1（HAND2-AS1）的表达。进行了为期 5 年的随访研究，以探讨 WTAPP1 对 NSCLC 的预后价值。进行过表达实验以分析 WTAPP1 和 HAND2-AS1 之间的相互作用。通过 Transwell 测定评估细胞侵袭和迁移。
结果
WTAPP1 在 NSCLC 中表达上调。生存分析表明，WTAPP1 血浆水平低与生存率高有关。HAND2-AS1 在 NSCLC 中下调，与肿瘤组织中的 WTAPP1 呈负相关。过表达 WTAPP1 导致 NSCLC 细胞中 HAND2-AS1 下调，而过表达 HAND2-AS1 不影响 WTAPP1 的表达。过表达 WTAPP1 导致 NSCLC 细胞侵袭和迁移增强，而过表达 HAND2-AS1 导致 NSCLC 细胞侵袭和迁移减弱。此外，过表达 HAND2-AS1 部分减弱了过表达 WTAPP1 的作用。此外，WTAPP1 不影响癌细胞增殖。
结论
WTAPP1 可能通过下调 lncRNA HAND2-AS1 促进 NSCLC 癌细胞侵袭和迁移。
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