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长链非编码 RNA GAS8-AS1 通过下调 lncRNA UCA1 抑制骨肉瘤细胞迁移和侵袭。

lncRNA GAS8-AS1 downregulates lncRNA UCA1 to inhibit osteosarcoma cell migration and invasion.

机构信息

Department of Orthopedics, Luoyang Orthopedic Hospital of Henan Province, Orthopedic Hospital of Henan Province, No.100 Yongping Road, Zhengzhou City, Henan Province, 450016, People's Republic of China.

Department of Orthopedics, Guangzhou University of Chinese Medicine Third Affiliated Hospital, Guangzhou City, Guangdong Province, 510375, People's Republic of China.

出版信息

J Orthop Surg Res. 2020 Feb 3;15(1):38. doi: 10.1186/s13018-020-1550-x.

DOI:10.1186/s13018-020-1550-x
PMID:32013985
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6998179/
Abstract

BACKGROUND

Osteosarcoma (OS) is the most common type of primary bone tumor that mainly affects adolescents and young adults. The present study explored the role of lncRNA GAS8-AS1 in OS.

METHODS

A total of 48 OS patients were selected from the 82 OS patients admitted by Luoyang Orthopedic Hospital of Henan Province between May 2010 and May 2013. Transient cell transfections, Transwell cell migration and invasion assay, RT-qPCR, and patient follow-up were carried out during the research.

RESULTS

The results showed that GAS8-AS1 was downregulated, while UCA1 was upregulate in cancer tissues in comparison to adjacent non-cancer tissues of OS patients. GAS8-AS1 was not affected by clinical stage. Follow-up study showed that downregulated GAS8-AS1 in cancer tissues was closely correlated with poor survival. GAS8-AS1 and UCA1 were inversely correlated in cancer tissues. Overexpression of UCA1 failed to affect the expression of GAS8-AS1, while overexpression of GAS8-AS1 led to downregulated expression of UCA1 in OS cells, while the molecular mediators between these two lncRNAs are unknown. Overexpression of GAS8-AS1 did not affect OS cell proliferation but significantly inhibited cancer cell migration and invasion. Overexpression of UCA1 promoted the migration and invasion of OS cells and attenuated the effects of overexpressing GAS8-AS1.

CONCLUSIONS

Therefore, GAS8-AS1 may inhibit OS cell migration and invasion by downregulating oncogenic UCA1.

摘要

背景

骨肉瘤（OS）是最常见的原发性骨肿瘤，主要影响青少年和年轻人。本研究探讨了长链非编码 RNA GAS8-AS1 在 OS 中的作用。

方法

从 2010 年 5 月至 2013 年 5 月河南省洛阳正骨医院收治的 82 例 OS 患者中选择 48 例 OS 患者。在研究过程中进行了瞬时细胞转染、Transwell 细胞迁移和侵袭试验、RT-qPCR 和患者随访。

结果

结果表明，与 OS 患者的癌旁非癌组织相比，GAS8-AS1 下调，而 UCA1 上调。GAS8-AS1 不受临床分期影响。随访研究表明，癌组织中下调的 GAS8-AS1 与不良生存密切相关。癌组织中 GAS8-AS1 与 UCA1 呈负相关。过表达 UCA1 未能影响 GAS8-AS1 的表达，而过表达 GAS8-AS1 导致 OS 细胞中 UCA1 的表达下调，而这两种 lncRNA 之间的分子介质尚不清楚。过表达 GAS8-AS1 不影响 OS 细胞增殖，但显著抑制癌细胞迁移和侵袭。过表达 UCA1 促进 OS 细胞的迁移和侵袭，并减弱过表达 GAS8-AS1 的作用。

结论

因此，GAS8-AS1 可能通过下调致癌 UCA1 抑制 OS 细胞的迁移和侵袭。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9bf/6998179/2819b546344f/13018_2020_1550_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9bf/6998179/a67817c0062e/13018_2020_1550_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9bf/6998179/8a6d5e849c26/13018_2020_1550_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9bf/6998179/c7f6f628b3c9/13018_2020_1550_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9bf/6998179/45c4d8f48834/13018_2020_1550_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9bf/6998179/2819b546344f/13018_2020_1550_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9bf/6998179/a67817c0062e/13018_2020_1550_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9bf/6998179/8a6d5e849c26/13018_2020_1550_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9bf/6998179/c7f6f628b3c9/13018_2020_1550_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9bf/6998179/45c4d8f48834/13018_2020_1550_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9bf/6998179/2819b546344f/13018_2020_1550_Fig5_HTML.jpg

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