Aix Marseille Université, IRD, AP-HM, MEФI, Institut Hospitalo-Universitaire Méditerranée-Infection, 19-21 Boulevard Jean Moulin, 13005, Marseille, France.
Pédiatrie Spécialisée Et Médecine Infantile, Hôpital de La Timone, AP-HM, Marseille, France.
Sci Rep. 2021 Jan 29;11(1):2684. doi: 10.1038/s41598-021-82420-z.
To date there are thirteen species validly assigned to the genus Anaerococcus. Most of the species in this genus are anaerobic and of human origin. Anaerococcus urinimassiliensis sp. nov., strain Marseille-P2143 is member of family Peptoniphilaceae, which was isolated from the urine of a 17-year-old boy affected by autoimmune hepatitis and membranoproliferative glomerulonephritis using the culturomic approach. In the current study, a taxono-genomics method was employed to describe this new species. The strain Marseille-P2143 was gram positive cocci with translucent colonies on blood agar. Its genome was 2,189,509 bp long with a 33.5 mol% G + C content and exhibited 98.48% 16S rRNA similarity with Anaerococcus provencensis strain 9,402,080. When Anaerococcus urinomassiliensis strain Marseill-P2143 is compared with closely related species, the values ranged from 71.23% with A. hydrogenalis strain DSM 7454 (NZ_ABXA01000052.1) to 90.64% with A. provencensis strain 9402080 (NZ_HG003688.1). This strain has implemented the repertoire of known bacteria of the human urinary tract.
迄今为止，已有 13 种有效归属到厌氧球菌属的种。该属的大多数种都是厌氧的，来源于人类。Anaerococcus urinimassiliensis sp. nov.，菌株 Marseille-P2143 是消化球菌科的成员，是通过培养组学方法从一名患有自身免疫性肝炎和膜增生性肾小球肾炎的 17 岁男孩的尿液中分离出来的。在本研究中，采用分类基因组学方法对这一新种进行了描述。Marseille-P2143 菌株是革兰阳性球菌，在血琼脂上形成半透明菌落。其基因组长 2,189,509 bp，G+C 含量为 33.5 mol%，与 Anaerococcus provencensis 菌株 9,402,080 的 16S rRNA 相似度为 98.48%。当 Anaerococcus urinomassiliensis 菌株 Marseill-P2143 与亲缘关系较近的种进行比较时，其值范围从与 A. hydrogenalis 菌株 DSM 7454（NZ_ABXA01000052.1）的 71.23%到与 A. provencensis 菌株 9402080（NZ_HG003688.1）的 90.64%。该菌株实现了人类泌尿道中已知细菌的库。
