Anaerococcus faecalis sp. nov., Isolated from Swine Faeces.

An obligate anaerobic, Gram-stain-positive, non-spore forming, non-motile, catalase and oxidase-negative, coccoid-shaped bacterium designated AGMB00486 was isolated from swine faeces. The optimal growth of the isolate occurred at pH 8.0 and 37 ℃. Furthermore, the growth was observed in the presence of up to 4% (w/v) NaCl but not at salinity levels higher than 5%. The phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain AGMB00486 was a member of the genus Anaerococcus and that the isolate was most closely related to Anaerococcus vaginalis KCTC 15028 (96.7% 16S rRNA gene sequence similarity) followed by Anaerococcus hydrogenalis KCTC 15014 (96.7%) and Anaerococcus senegalensis KCTC 15435 (96.3%). Whole-genome sequence analysis determined that the DNA G+C content of strain AGMB00486 was 30.1 mol%, and the genome size, numbers of tRNA and rRNA genes were 2,268,866 bp, 47 and 8, respectively. The average nucleotide identity values between strain AGMB00486 and the three related type strains were 77.0, 77.4 and 77.2%, respectively. The major cellular fatty acids (> 10%) of strain AGMB00486 were C, C and C DMA. Accordingly, these distinct phenotypic and phylogenetic properties revealed that strain AGMB00486 represents a novel species, for which the name Anaerococcus faecalis sp. nov. is proposed. The type strain is AGMB00486 (= KCTC 15945 = CCTCC AB 202009).