Department of Biological Sciences, University of Maryland Baltimore County, Baltimore, MD, 21250, USA.
Commun Biol. 2021 Jan 29;4(1):147. doi: 10.1038/s42003-021-01655-8.
Primary neurulation is the process by which the neural tube, the central nervous system precursor, is formed from the neural plate. Incomplete neural tube closure occurs frequently, yet underlying causes remain poorly understood. Developmental studies in amniotes and amphibians have identified hingepoint and neural fold formation as key morphogenetic events and hallmarks of primary neurulation, the disruption of which causes neural tube defects. In contrast, the mode of neurulation in teleosts has remained highly debated. Teleosts are thought to have evolved a unique mode of neurulation, whereby the neural plate infolds in absence of hingepoints and neural folds, at least in the hindbrain/trunk where it has been studied. Using high-resolution imaging and time-lapse microscopy, we show here the presence of these morphological landmarks in the zebrafish anterior neural plate. These results reveal similarities between neurulation in teleosts and other vertebrates and hence the suitability of zebrafish to understand human neurulation.
初级神经胚形成是神经管（中枢神经系统前体）从神经板形成的过程。不完全的神经管闭合经常发生，但潜在的原因仍知之甚少。羊膜动物和两栖动物的发育研究已经确定了铰链点和神经褶形成是初级神经胚形成的关键形态发生事件和标志，它们的破坏会导致神经管缺陷。相比之下，硬骨鱼的神经胚形成模式仍然存在很大争议。硬骨鱼被认为进化出了一种独特的神经胚形成模式，至少在已经研究过的后脑/躯干中，神经板在没有铰链点和神经褶的情况下内陷。通过高分辨率成像和延时显微镜，我们在这里显示了斑马鱼前神经板中存在这些形态学标志。这些结果揭示了硬骨鱼和其他脊椎动物的神经胚形成之间的相似性，因此斑马鱼适合用来理解人类的神经胚形成。
