Araya Claudio, Carmona-Fontaine Carlos, Clarke Jonathan D W
Laboratory of Developmental Biology, Instituto de Ciencias Marinas y Limnológicas, Facultad de Ciencias, Universidad Austral de Chile, Campus Isla Teja s/n, Valdivia, Chile.
MRC Centre for Developmental Neurobiology, King's College London, New Hunt's House, 4th Floor, Guy's Hospital Campus, London, United Kingdom.
Dev Dyn. 2016 May;245(5):580-9. doi: 10.1002/dvdy.24401. Epub 2016 Apr 6.
During the initial stages zebrafish neurulation, neural plate cells undergo highly coordinated movements before they assemble into a multicellular solid neural rod. We have previously identified that the underlying mesoderm is critical to ensure such coordination and generate correct neural tube organization. However, how intertissue coordination is achieved in vivo during zebrafish neural tube morphogenesis is unknown.
In this work, we use quantitative live imaging to study the coordinated movements of neural ectoderm and mesoderm during dorsal tissue convergence. We show the extracellular matrix components laminin and fibronectin that lie between mesoderm and neural plate act to couple the movements of neural plate and mesoderm during early stages of neurulation and to maintain the close apposition of these two tissues.
Our study highlights the importance of the extracellular matrix proteins laminin and libronectin in coupling the movements and spatial proximity of mesoderm and neuroectoderm during the morphogenetic movements of neurulation. Developmental Dynamics 245:580-589, 2016. © 2016 Wiley Periodicals, Inc.
在斑马鱼神经胚形成的初始阶段，神经板细胞在组装成多细胞实心神经杆之前会经历高度协调的运动。我们之前已经确定，其下方的中胚层对于确保这种协调并产生正确的神经管组织至关重要。然而，在斑马鱼神经管形态发生过程中，体内组织间的协调是如何实现的尚不清楚。
在这项研究中，我们使用定量实时成像技术来研究背侧组织汇聚过程中神经外胚层和中胚层的协调运动。我们发现，位于中胚层和神经板之间的细胞外基质成分层粘连蛋白和纤连蛋白，在神经胚形成的早期阶段起到了耦合神经板和中胚层运动的作用，并维持这两个组织的紧密贴合。
我们的研究强调了细胞外基质蛋白层粘连蛋白和纤连蛋白在神经胚形成的形态发生运动过程中，耦合中胚层和神经外胚层运动以及空间接近性方面的重要性。《发育动力学》2016年第245卷，第580 - 589页。©版权所有2016年威利期刊公司。
