Atar Necip, Yola Mehmet Lütfi
Pamukkale University, Faculty of Engineering, Department of Chemical Engineering, Denizli, Turkey.
Hasan Kalyoncu University, Faculty of Health Sciences, Department of Nutrition and Dietetics, Gaziantep, Turkey.
Anal Chim Acta. 2021 Mar 1;1148:338202. doi: 10.1016/j.aca.2021.338202. Epub 2021 Jan 7.
Interleukin 6 (IL-6) acts as both a proinflammatory and anti-inflammatory cytokine and is generally utilized as an important diagnostic biomarker for sepsis. In addition, the high levels of IL-6 measured in plasma have been associated with pathological inflammation. A novel quartz crystal microbalance (QCM) immunoassay method was presented for high sensitivity and selectivity detection of interleukin-6 (IL-6) based on gold nanoparticles functionalized sulfur-doped graphene quantum dot (AuNPs/S-GQD) and hollow ZnS-CdS nanocage (h-ZnS-CdS NC). Firstly, AuNPs/S-GQD nanocomposite was synthesized in the presence of tetrachloroauric acid and then conjugated onto anti-IL-6 antibodies by amino-gold affinity. The sandwich-type QCM immunoassay probe was prepared by immune-reaction between AuNPs/S-GQD/QCM immobilized with anti-IL-6 capture antibodies and h-ZnS-CdS NC including detection anti-IL-6 antibodies in the presence of target IL-6. The prepared QCM immunoassay probe was characterized by transmission electron microscopy (TEM), scanning electron microscope (SEM), x-ray diffraction (XRD) method, x-ray photoelectron spectroscopy (XPS), Raman spectroscopy, UV-vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The QCM immunosensor showed a linearity range (0.01-2.0 pg mL) and a low detection limit (3.33 fg mL). Lastly, high stable and selective QCM immunosensor was applied to prepared plasma samples with good recovery.
白细胞介素6（IL-6）兼具促炎和抗炎细胞因子的作用，通常被用作脓毒症的重要诊断生物标志物。此外，血浆中检测到的高水平IL-6与病理性炎症有关。基于金纳米颗粒功能化硫掺杂石墨烯量子点（AuNPs/S-GQD）和中空硫化锌-硫化镉纳米笼（h-ZnS-CdS NC），提出了一种用于高灵敏度和选择性检测白细胞介素6（IL-6）的新型石英晶体微天平（QCM）免疫分析方法。首先，在氯金酸存在下合成AuNPs/S-GQD纳米复合材料，然后通过氨基-金亲和力将其偶联到抗IL-6抗体上。通过将固定有抗IL-6捕获抗体的AuNPs/S-GQD/QCM与在目标IL-6存在下包含检测抗IL-6抗体的h-ZnS-CdS NC进行免疫反应，制备夹心型QCM免疫分析探针。通过透射电子显微镜（TEM）、扫描电子显微镜（SEM）、X射线衍射（XRD）方法、X射线光电子能谱（XPS）、拉曼光谱、紫外-可见光谱、傅里叶变换红外光谱（FTIR）、循环伏安法（CV）和电化学阻抗谱（EIS）对制备的QCM免疫分析探针进行表征。该QCM免疫传感器显示出线性范围（0.01 - 2.0 pg/mL）和低检测限（3.33 fg/mL）。最后，将高稳定性和选择性的QCM免疫传感器应用于制备的血浆样品，回收率良好。
