Graduate School, Guangxi University of Chinese Medicine, Nanning 530000, China.
Graduate School, Guangxi Medical University, Nanning 530000, China.
Chin J Nat Med. 2021 Jan;19(1):28-35. doi: 10.1016/S1875-5364(21)60003-1.
Osteoporosis is a health problem to cause global concern. A lot of methods have been used to prevent and treat osteoporosis, but there is still a lack of effective treatment for osteoporosis owing to limited understanding of its mechanism. Therefore, the aim of this present study is to explore the underlying mechanism of Wuling Powder, a traditional Chinese medicine on treating osteoporosis. In this study, we firstly screened and identified the common targets between Wuling Powder and osteoporosis through the related databases, and then explored the relationships among these targets, Wuling Powder and osteoporosis by using Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and network analyses. Subsequently, the molecular docking was performed by using systemsDock to evaluate the potential binding relationships between the active components of Wuling Powder and their related targets. The results showed that in total of 14 common targets including CREBBP, ADAM17, GOT1, GAPDH, USP8, ERBB2, EEF1A1, MTOR, RAC1, ETS1, DDX58, GCK, EGF and S100A8 were screened. EGF, ERBB2, MTOR and HIF-1 were the potential therapeutic targets for osteoporosis, and they were also the related targets for predicting active components in Wuling Powder. Taken together, we concluded that Wuling Powder might be used to treat osteoporosis through above these targets.
骨质疏松症是一个引起全球关注的健康问题。已经有很多方法被用于预防和治疗骨质疏松症，但由于对其机制的理解有限，仍然缺乏有效的治疗方法。因此，本研究旨在探索五灵散治疗骨质疏松症的潜在机制。在本研究中，我们首先通过相关数据库筛选和鉴定五灵散与骨质疏松症之间的共同靶点，然后通过基因本体论（GO）、京都基因与基因组百科全书（KEGG）和网络分析探讨这些靶点、五灵散与骨质疏松症之间的关系。随后，通过系统 docking 进行分子对接，评估五灵散活性成分与其相关靶点之间的潜在结合关系。结果表明，共筛选出包括 CREBBP、ADAM17、GOT1、GAPDH、USP8、ERBB2、EEF1A1、MTOR、RAC1、ETS1、DDX58、GCK、EGF 和 S100A8 在内的 14 个共同靶点。EGF、ERBB2、MTOR 和 HIF-1 是骨质疏松症的潜在治疗靶点，也是预测五灵散中活性成分的相关靶点。综上所述，我们得出结论，五灵散可能通过上述靶点治疗骨质疏松症。
