Laboratorio de Toxicología Molecular, Centro de Investigación Aplicada en Ambiente y Salud (CIAAS), Coordinación para la Innovación y Aplicación de la Ciencia y la Tecnología (CIACYT), Universidad Autónoma de San Luis Potosí, San Luis Potosí, Mexico; Facultad de Medicina, Universidad Autónoma de San Luis Potosí, San Luis Potosí, Mexico.
Facultad de Enfermería, Universidad Autónoma de Zacatecas, Zacatecas, Zacatecas, Mexico.
Environ Toxicol Pharmacol. 2021 Apr;83:103598. doi: 10.1016/j.etap.2021.103598. Epub 2021 Jan 28.
The environmental contamination with lead (Pb) is considered a critical issue worldwide. Therefore, this study aimed to evaluate the expression levels of circulating miRNAs (miR-155, miR-126, and miR-145) in Mexican women exposed to Pb. Blood lead levels (BLL) were assessed in enrolled women (n = 190) using an atomic absorption method. Also, serum miRNAs expression levels were quantified through a real-time PCR assay. A mean BLL of 10.5 ± 4.50 μg/dL was detected. Overexpression of miR-155 was detected in highly exposed women. Besides, a significant simple positive relationship (p < 0.05) was found between BLL and serum miR-155 expression levels. Additionally, a significant inverse correlation (p < 0.05) was determined between BLL and serum miR-126 expression levels, as downregulation of miR-126 expression levels was observed in highly exposed women. The findings in this study are the concern, as epigenetic changes detected may represent a connection between health illnesses and Pb exposure.
环境铅（Pb）污染被认为是一个全球性的关键问题。因此，本研究旨在评估暴露于 Pb 的墨西哥妇女循环 miRNA（miR-155、miR-126 和 miR-145）的表达水平。通过原子吸收法评估纳入妇女（n=190）的血铅水平（BLL）。还通过实时 PCR 测定定量血清 miRNA 表达水平。检测到平均 BLL 为 10.5±4.50μg/dL。高暴露组检测到 miR-155 的过表达。此外，还发现 BLL 与血清 miR-155 表达水平之间存在显著的简单正相关（p<0.05）。此外，还确定了 BLL 与血清 miR-126 表达水平之间存在显著的负相关（p<0.05），因为在高暴露组中观察到 miR-126 表达水平下调。本研究的发现令人担忧，因为检测到的表观遗传变化可能代表健康疾病与 Pb 暴露之间的联系。
