Zhu Feng, Wu Xiaosong, Li Fang, Wang Wei, Ji Wenliang, Liu Hualiang, Xu Yan
Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China.
Wei Sheng Yan Jiu. 2021 Jan;50(1):121-128. doi: 10.19813/j.cnki.weishengyanjiu.2021.01.021.
To develop a method for the determination of 13 antibiotics in 8 classes for desinfection products by ulta-high perfomance chromatography-tandem mass spectrometry(UPLC-MS/MS).
Samples were extracted by methanol or acetonitrile. The target compouds were separated on a Waters HSS T3 column(100 mm×2. 1 mm, 1. 8 μm), and detected by triple quadrupole tandem mass spectrometer.
The 13 selected antibiotics showed good linear relationships in the range of 4-100 μg/L and the correlation coefficients(r~2) were all above 0. 991. The limits of detection ranged from 2 to 25 μg/kg. The recovery rates at three spiked levels(low, medium and high) in three dosage forms of disinfection products were in the range of 71. 2%-130. 4%, and the relative standard deviations(RSD) were all less than 11. 3%, which could meet the detection requirements of illegal addition of antibiotics in disinfection products. Ofloxacin at a concentration of 21. 1 mg/kg was found in a cream disinfection product by the developed method, and no related drugs were detected in other samples.
This method is simple, reliable, reproducible, which covers a wide range of antibiotics, and provides technical support for monitoring the illegal addition of antibiotics in disinfection products.
