Key Laboratory of Plant Protection Resources & Pest Management of the Ministry of Education, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China.
Key Laboratory of Plant Protection Resources & Pest Management of the Ministry of Education, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China; Department of Entomology, UCA&ES, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.
Pestic Biochem Physiol. 2021 Feb;172:104769. doi: 10.1016/j.pestbp.2020.104769. Epub 2021 Jan 4.
Cantharidin (CTD) is a natural toxin with effective toxicity to lepidopteran pests. Nevertheless, little information is available on whether pests develop resistance to CTD. After being exposed to CTD (50 mg/L to 90 mg/L) or 10 generations, the resistance ratio of laboratory selected cantharidin-resistant Mythimna separata (Cantharidin-SEL) strain was only elevated 1.95-fold. Meanwhile, the developmental time for M. separata was prolonged (delayed1.65 in males and 1.84 days in females). The reported CTD target, the serine/threonine phosphatases (PSPs), have not been shown significant activity variation during the whole process of CTD-treatment. The activity of detoxification enzymes (cytochrome monooxygenase P450, glutathione-S-transferase (GST) and carboxylesterase) were affected by CTD selection, but this change was not mathematically significant. More importantly, no obvious cross-resistance with other commonly used insecticides was observed in the M. separata population treated with CTD for 10 generations (resistance ratios were all lower 2.5). Overall, M. separata is unlikely to produce target-site insensitivity resistance, metabolic resistance to CTD. Meanwhile, cantharidin-SEL is not prone to develop cross-resistance with other insecticides. These results indicate that CTD is a promising biogenetic lead compound which can be applied in the resistance management on M. separata.
斑蝥素（CTD）是一种对鳞翅目害虫具有有效毒性的天然毒素。然而，关于害虫是否对 CTD 产生抗性的信息却很少。经过 CTD（50mg/L 至 90mg/L）或 10 代的暴露，实验室选择的抗斑蝥素抗性斜纹夜蛾（Cantharidin-SEL）品系的抗性比仅提高了 1.95 倍。同时，斜纹夜蛾的发育时间延长（雄性延迟 1.65 天，雌性延迟 1.84 天）。报道的 CTD 靶标丝氨酸/苏氨酸磷酸酶（PSPs）在整个 CTD 处理过程中未显示出明显的活性变化。解毒酶（细胞色素单加氧酶 P450、谷胱甘肽-S-转移酶（GST）和羧酸酯酶）的活性受到 CTD 选择的影响，但这种变化在数学上并不显著。更重要的是，在经过 10 代 CTD 处理的斜纹夜蛾种群中，没有观察到与其他常用杀虫剂的明显交叉抗性（抗性比均低于 2.5）。总体而言，斜纹夜蛾不太可能对 CTD 产生靶标不敏感性抗性或代谢抗性。同时，抗斑蝥素斜纹夜蛾不易对其他杀虫剂产生交叉抗性。这些结果表明，CTD 是一种很有前途的生物遗传先导化合物，可应用于斜纹夜蛾的抗药性管理。
