Fan Qiqi, Liu Jiyuan, Li Yifan, Zhang Yalin
Key Laboratory of Plant Protection Resources and Pest Management, Ministry of Education, Entomological Museum, College of Plant Protection, Northwest A&F University, Yangling, Xianyang 712100, China.
Insects. 2022 Oct 28;13(11):989. doi: 10.3390/insects13110989.
The control of primarily involves chemical insecticides, but overuse has brought about many negative effects. Our previous study reported that (S)-(-)-palasonin (PLN) is a plant-derived active substance with significant insecticidal activity against . However, we noticed a possible cross-resistance between (S)-(-)-palasonin and other insecticides which may be related to metabolic detoxification. In order to further explore the detoxification effect of detoxification enzymes on (S)-(-)-palasonin in , the effects of (S)-(-)-palasonin on enzyme activity and transcription level were determined, and the detoxification and metabolism of GSTs on (S)-(-)-palasonin were studied by in vitro inhibition and metabolism experiments. During this study, GST enzyme activity was significantly increased in after (S)-(-)-palasonin treatment. The expression levels of 19 GSTs genes were significantly increased whereas the expression levels of 1 gene decreased. Furthermore, (S)-(-)-palasonin is shown to be stabilized with GSTs and metabolized GSTs (GSTd1, GSTd2, GSTs1 and GSTs2) in vitro, with the highest metabolic rate of 80.59% for GSTs1. This study advances the beneficial utilization of (S)-(-)-palasonin as a botanical pesticide to control in the field.
对[害虫名称未给出]的防治主要涉及化学杀虫剂，但过度使用已带来许多负面影响。我们之前的研究报告称，(S)-(-)-帕拉索宁（PLN）是一种植物源活性物质，对[害虫名称未给出]具有显著的杀虫活性。然而，我们注意到(S)-(-)-帕拉索宁与其他杀虫剂之间可能存在交叉抗性，这可能与代谢解毒有关。为了进一步探究解毒酶对[害虫名称未给出]体内(S)-(-)-帕拉索宁的解毒作用，测定了(S)-(-)-帕拉索宁对酶活性和转录水平的影响，并通过体外抑制和代谢实验研究了谷胱甘肽S-转移酶（GSTs）对(S)-(-)-帕拉索宁的解毒和代谢作用。在这项研究中，经(S)-(-)-帕拉索宁处理后，[害虫名称未给出]体内的GST酶活性显著增加。19个GSTs基因的表达水平显著升高，而1个基因的表达水平下降。此外，(S)-(-)-帕拉索宁在体外与GSTs结合并被GSTs（GSTd1、GSTd2、GSTs1和GSTs2）代谢，其中GSTs1的最高代谢率为80.59%。本研究推动了(S)-(-)-帕拉索宁作为一种植物源杀虫剂在田间防治[害虫名称未给出]的有益利用。
