Institute of Zoology, University of the Punjab, Lahore, Pakistan.
Parasitol Res. 2024 Mar 9;123(3):157. doi: 10.1007/s00436-024-08180-7.
Musca domestica Linnaeus is a devastating insect pest of medical and veterinary importance with reports of resistance development to commonly used insecticides worldwide. Rearing substrates usually play a crucial role in determining susceptibility to insecticides and control of insect pests. The aim of the present study was to investigate the effect of five rearing substrates of M. domestica on its susceptibility to different insecticides and activities of metabolic enzymes. After 30 generations of rearing, susceptibility of M. domestica to tested insecticides, viz., malathion, pirimiphos-methyl, alpha-cypermethrin, deltamethrin, methomyl, propoxur, spinetoram, and chlorfenapyr had evident differences. Musca domestica reared on hen liver exhibited reduced susceptibility to all insecticides followed by the strain reared on poultry manure. However, M. domestica reared on milk-based diet showed the highest susceptibility to tested insecticides followed by the strain reared on manures of buffalo and horse. In addition, M. domestica reared on different substrates exhibited significant differences (p < 0.01) in the activities of glutathione S-transferase (GST), cytochrome P450-dependent monooxygenase, and carboxylesterase (CarE). Overall, hen liver and poultry manure strains exhibited higher activities of metabolic enzymes than those of the milk-based diet, buffalo, and horse manure strains. In conclusion, the data of the present study exhibited a significant effect of rearing substrates on the susceptibility to insecticides and activities of metabolic enzymes in M. domestica. These results could be helpful for the sustainable management of M. domestica on different hosts by selecting appropriate insecticides.
家蝇是一种具有医学和兽医学重要意义的毁灭性昆虫害虫，全世界都有报道称其对常用杀虫剂产生了抗性。饲养基质通常在决定对杀虫剂的敏感性和控制害虫方面起着至关重要的作用。本研究旨在调查家蝇的五种饲养基质对其对不同杀虫剂敏感性和代谢酶活性的影响。经过 30 代的饲养，家蝇对测试杀虫剂（马拉硫磷、丙溴磷、α-氯氰菊酯、溴氰菊酯、灭多威、残杀威、螺虫乙酯和氯氟吡氧乙酸）的敏感性有明显差异。在家蝇肝脏饲养的家蝇对所有杀虫剂的敏感性降低，其次是在家禽粪便中饲养的家蝇。然而，在家畜奶基饲养的家蝇对测试杀虫剂的敏感性最高，其次是在家畜粪便中饲养的家蝇。此外，在家蝇不同基质上饲养的家蝇在谷胱甘肽 S-转移酶（GST）、细胞色素 P450 依赖性单加氧酶和羧酸酯酶（CarE）的活性方面表现出显著差异（p < 0.01）。总体而言，肝脏和家禽粪便饲养的家蝇表现出比奶基饲养、水牛和马粪饲养的家蝇更高的代谢酶活性。总之，本研究的数据表明饲养基质对家蝇对杀虫剂的敏感性和代谢酶活性有显著影响。这些结果有助于通过选择合适的杀虫剂来实现对不同宿主家蝇的可持续管理。
