Zhu Zhihao, Huang Bingbing, Sun Ningning, Yu Xuanci, Du Ziyuan, Li Ang, Huang Caiyun
College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Jiangxi Functional Feed Additive Engineering Laboratory, Institute of Biological Resource, Jiangxi Academy of Sciences, Nanchang, Jiangxi 330096, China.
Poult Sci. 2024 Dec;103(12):104399. doi: 10.1016/j.psj.2024.104399. Epub 2024 Oct 10.
High broodiness in Muscovy ducks impedes animal husbandry growth. The interaction between endocrine hormones and gut microbiota has been proven to play a crucial role in reproductive performance, and whether it can regulate the broody behavior of Muscovy ducks requires further research. Nine laying ducks (Laying group) and nine broody ducks (Broodiness group) were selected. Corresponding serum, ileum, and cecum chyme were collected for further research. The results showed that, compared to the laying group, the serum concentration of prolactin decreased, while the levels of Mullerian inhibiting substance, follicle-stimulating hormone, and follistatin increased in the broodiness group (P < 0.05). 16S rDNA sequencing showed that, the broodiness group exhibited lower abundance levels of Rothia, Streptococcus, and Lactobacillus, whereas the abundance of Turicibacter, Aliicoccus, and Facklamia was higher in the ileum compared to the laying group (P < 0.05). In the cecum, the broodiness group exhibits a significant reduction in the abundance of Butyricicoccus and unclassified_f_Rikenellaceae, while the abundance of Christensenellaceae_R-7_group, Ruminococcus_torques_group, Parabacteroides, norank_f_Oscillospiraceae, Cloacibacillus, Sellimonas, Shuttleworthia, norank_f_UCG-010, unclassified_f_Lachnospiraceae, Oscillospira, Synergistes, Family_XIII_AD3011_group and Eubacterium_nodatum_group is higher compared to the laying group. A Spearman correlation analysis reveals that both in the ileum and cecum, serum hormones exhibit significant correlations with the top 20 abundant intestinal microbial genera. Among these, serum follistatin has most entries of significant correlations with the detected microbial genera (P < 0.05). In conclusion, the broody behavior of Muscovy ducks can be modulated by the interaction between hormones and gut microbiota. Notably, the relationship between Follistatin and the composition of gut microbiota, specifically Firmicutes, is the most prominent.
番鸭的高就巢性阻碍了畜牧业的发展。内分泌激素与肠道微生物群之间的相互作用已被证明在繁殖性能中起着关键作用，而其是否能调节番鸭的就巢行为尚需进一步研究。选取9只产蛋鸭（产蛋组）和9只就巢鸭（就巢组）。采集相应的血清、回肠和盲肠食糜用于进一步研究。结果表明，与产蛋组相比，就巢组催乳素血清浓度降低，而苗勒氏管抑制物质、促卵泡激素和卵泡抑素水平升高（P<0.05）。16S rDNA测序显示，就巢组回肠中罗氏菌属、链球菌属和乳杆菌属的丰度水平较低，而与产蛋组相比，图里西菌属、阿利球菌属和法克勒菌属的丰度较高（P<0.05）。在盲肠中，就巢组丁酸球菌属和未分类的理研菌科丰度显著降低，而与产蛋组相比，克里斯滕森菌科_R-7_组、扭链瘤胃球菌组、副拟杆菌属、未分类的颤螺菌科、泄殖腔杆菌属、塞尔利莫纳斯菌属、沙特尔沃思菌属、未分类的_UCG-010、未分类的毛螺菌科、颤螺菌属、协同菌属、XIII_AD3011_科和瘤胃真杆菌组的丰度较高。Spearman相关性分析显示，在回肠和盲肠中，血清激素与肠道微生物丰度排名前20的菌属均存在显著相关性。其中，血清卵泡抑素与检测到的微生物菌属显著相关的条目最多（P<0.05）。综上所述，番鸭的就巢行为可通过激素与肠道微生物群之间的相互作用来调节。值得注意的是，卵泡抑素与肠道微生物群组成之间的关系，特别是与厚壁菌门的关系最为突出。
