Kishk Rania, Soliman Nourhan, Nemr Nader, Eldesouki Raghda, Mahrous Nageh, Gobouri Adil, Azab Ehab, Anani Maha
Microbiology and Immunology Department, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.
Clinical Pathology Department, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.
Infect Drug Resist. 2021 Jan 19;14:143-150. doi: 10.2147/IDR.S290584. eCollection 2021.
is an opportunistic pathogen that rapidly develops antibiotic resistance against commonly prescribed antimicrobial agents in hospitalized patients worldwide. Aminoglycosides are commonly used in the treatment of health care-associated infections (HAIs). Aminoglycosides resistance mechanisms are varied and commonly involve production of aminoglycoside-modifying enzymes (AME) and efflux systems.
This study aimed to provide an insight into the frequency of genes encoding AME in strains isolated from different clinical specimens in intensive care units (ICU).
A total of 52 multidrug-resistant (MDR) strains were isolated from ICU, Suez Canal University Hospitals. Species identification and antibiotics susceptibility testing were done by the automated system VITEK 2. The genes encoding AME were detected by PCR.
Aminoglycosides resistance (amikacin, gentamicin and tobramycin) was observed in 35 isolates (67.3%). We found that aacC1 gene was the predominant AME resistance gene among isolates, detected in 14 isolates (40%), aphA6 in 11 isolates (31.4%) and addA1 in 5 isolates (14.2%). We found 5 isolates containing 2 AME genes, 3 of them with aacC1 and aphA6 and the remaining 2 with both aacC1 and aadA1 genes. Nearly, 5 isolates (14.2%) were negative for all AME resistance genes.
Our study indicated that AME encoding genes are predominant in strains in our region which stressed on the importance of preventive measures to control spreading of resistance genes.
是一种机会致病菌，在全球范围内的住院患者中，它会迅速对常用的抗菌药物产生耐药性。氨基糖苷类药物常用于治疗医疗保健相关感染（HAIs）。氨基糖苷类耐药机制多种多样，通常涉及氨基糖苷类修饰酶（AME）的产生和外排系统。
本研究旨在深入了解重症监护病房（ICU）中从不同临床标本分离出的菌株中编码AME的基因频率。
从苏伊士运河大学医院的ICU共分离出52株多重耐药（MDR）菌株。通过VITEK 2自动化系统进行菌种鉴定和抗生素敏感性测试。通过PCR检测编码AME的基因。
在35株分离株（67.3%）中观察到对氨基糖苷类药物（阿米卡星、庆大霉素和妥布霉素）耐药。我们发现aacC1基因是分离株中主要的AME耐药基因，在14株（40%）中检测到，aphA6在11株（31.4%）中检测到，addA1在5株（14.2%）中检测到。我们发现5株分离株含有2个AME基因，其中3株同时含有aacC1和aphA6，其余2株同时含有aacC1和aadA1基因。近5株（14.2%）对所有AME耐药基因均为阴性。
我们的研究表明，编码AME的基因在我们地区的菌株中占主导地位，这强调了采取预防措施控制耐药基因传播的重要性。
