Tahara Yutaka, Obara Katsuya
Chiba Prefectural Oihama High School, Shioda-cho, Chuo-ku, Chiba 260-0823, Japan.
Takanedai Animal Clinic, Narashinodai, Funabashi 274-0063, Japan.
J Poult Sci. 2021 Jan 25;58(1):1-4. doi: 10.2141/jpsa.0200016.
culture of avian embryos can be applied not only to embryology but also to various fields of basic research such as embryo manipulation, toxicology, and regenerative medicine. The windowing method, which facilitates various manipulations and observations by opening a hole in one part of the eggshell, and culture systems using surrogate eggshells, are widely used. Despite this, biology lessons in high schools cover shell-less culture systems, which involve the development of avian embryos in artificial vessels, such as rice bowls, without using surrogate eggshells. However, as embryo development stops at its early stages in this method, it is not possible to continuously observe the development of the embryo. This led to attempts to develop an embryo culture method using a complete artificial culture vessel that does not use surrogate eggshells, and Kamihira (1998) succeeded in hatching quail embryos in an artificial culture vessel using polytetrafluoroethylene membranes. In addition, Tahara succeeded in hatching chick embryos in artificial culture vessels that used cling film made of polymethylpentene and reported their detailed methodology (Tahara and Obara, 2014). These technologies are being applied not only to school education but also to various fields of research.
禽类胚胎培养不仅可应用于胚胎学，还可应用于胚胎操作、毒理学和再生医学等基础研究的各个领域。开窗法通过在蛋壳的一部分开一个洞来方便各种操作和观察，以及使用替代蛋壳的培养系统，都被广泛使用。尽管如此，高中生物课讲授的是无壳培养系统，即禽类胚胎在诸如饭碗等人工容器中发育，而不使用替代蛋壳。然而，由于在这种方法中胚胎发育在早期阶段就会停止，因此无法持续观察胚胎的发育情况。这促使人们尝试开发一种不使用替代蛋壳的完整人工培养容器的胚胎培养方法，Kamihira（1,998年）成功地在使用聚四氟乙烯膜的人工培养容器中孵化出鹌鹑胚胎。此外，Tahara成功地在使用聚甲基戊烯制成的保鲜膜的人工培养容器中孵化出鸡胚胎，并报告了其详细方法（Tahara和Obara，2014年）。这些技术不仅应用于学校教育，还应用于各个研究领域。
