Kamihira M, Oguchi S, Tachibana A, Kitagawa Y, Iijima S
Department of Biotechnology, Graduate School of Engineering, Nagoya University, Japan.
Dev Growth Differ. 1998 Aug;40(4):449-55. doi: 10.1046/j.1440-169x.1998.t01-2-00010.x.
The establishment of avian embryonic culture is important both for the analysis of the developmental process and the establishment of transgenic chickens that produce useful biological materials in eggs. However, the hatchability of cultured embryos has been approximately 50%. We identified that the low rate of hatchability of cultured embryos was caused by limited oxygen and calcium availability. In quail embryo culture using chicken eggshell as a culture vessel, viability in the middle stage of culture was improved and 30% of embryos were hatched by oxygen enrichment. Furthermore, hatchability increased to 80% by supplementation with calcium lactate in addition to oxygen aeration. In the present study, a fully artificial vessel for quail embryo culture was designed using a gas-permeable Teflon membrane. By the addition of fine eggshell powder and calcium lactate, quail embryos grew and developed normally, and 43% of embryos hatched. Although the hatchability was lower than that of cultures using a surrogate eggshell, we achieved in hatching an avian embryo using a fully artificial vessel.
鸟类胚胎培养体系的建立对于分析发育过程以及培育能在蛋中产生有用生物材料的转基因鸡均具有重要意义。然而，培养胚胎的孵化率约为50%。我们发现，培养胚胎孵化率低是由于氧气供应和钙供应受限所致。在用鸡蛋壳作为培养容器的鹌鹑胚胎培养中，培养中期的存活率得到提高，通过富氧处理，30%的胚胎孵化。此外，除了通气供氧外，添加乳酸钙后孵化率提高到了80%。在本研究中，使用透气的聚四氟乙烯膜设计了一种用于鹌鹑胚胎培养的全人工培养容器。通过添加细蛋壳粉和乳酸钙，鹌鹑胚胎正常生长发育，43%的胚胎孵化。虽然孵化率低于使用替代蛋壳培养的情况，但我们成功地利用全人工培养容器孵化了鸟类胚胎。
