College of Medical Laboratory Sciences, University of Khartoum, Khartoum, Sudan.
Department of Basic Medical Sciences, Microbiology Unit, College of Medicine, University of Bisha, Bisha, Kingdom of Saudi Arabia.
Pan Afr Med J. 2020 Nov 3;37:213. doi: 10.11604/pamj.2020.37.213.24988. eCollection 2020.
the emergence of antibiotic resistance pathogens is an important health risk. Usually Gram negative bacteria acquire resistance to beta-lactam antibiotics by beta-lactamase production. The objectives of this study was to assess the prevalence of ESBL and to detect the frequency of blaTEM, blaSHV and blaCTX-M genotypes among ESBL producing Enterobacteriaceae isolates from patients in Khartoum, Sudan.
a total of 171 isolates of Enterobacteriaceae were recovered from hospitals in Khartoum, Sudan (2014 -2015) were used to detect ESBL production using disc diffusion method. blaTEM, blaSHV and blaCTX-M genes were investigated by PCR based methods using gene-specific primers.
the high resistance among Enterobacteriaceae was noticed in ciprofloxacin (72%) and ofloxacin (73%). ESBL production was mainly in Escherichia Coli (38%) and Klebsiella pneumonia (34%). Prevalent genotypes were blaTEM (86%), blaCTX-M (78%) and blaSHV (28%). These were found mainly in Escherichia Coli (38%, 37%, 2%) and K. pneumonia (34%, 31%, 26.1%). The majority of ESBL producing isolates possess more than one ESBL genes.
the ESBL production in Enterobacteriaceae was high, with blaTEM and blaCTX-M genotypes more prevalent. Public health and laboratory standard of excellence is needed to reducing the spread of resistant pathogens.
抗生素耐药病原体的出现是一个重要的健康风险。通常，革兰氏阴性细菌通过产生β-内酰胺酶来获得对β-内酰胺类抗生素的耐药性。本研究的目的是评估 ESBL 的流行率，并检测苏丹喀土穆患者中产 ESBL 的肠杆菌科分离株中 blaTEM、blaSHV 和 blaCTX-M 基因型的频率。
从苏丹喀土穆的医院中回收了 171 株肠杆菌科分离株（2014-2015 年），使用纸片扩散法检测 ESBL 的产生。使用基因特异性引物通过 PCR 方法检测 blaTEM、blaSHV 和 blaCTX-M 基因。
肠杆菌科对环丙沙星（72%）和氧氟沙星（73%）的耐药率较高。ESBL 主要产生于大肠杆菌（38%）和肺炎克雷伯菌（34%）。流行的基因型是 blaTEM（86%）、blaCTX-M（78%）和 blaSHV（28%）。这些主要存在于大肠杆菌（38%、37%、2%）和肺炎克雷伯菌（34%、31%、26.1%）中。大多数产 ESBL 的分离株携带一种以上的 ESBL 基因。
肠杆菌科中 ESBL 的产生率很高，blaTEM 和 blaCTX-M 基因型更为普遍。需要公共卫生和卓越实验室标准来减少耐药病原体的传播。
