Li Min, Zhao Lanbo, Zhou Xue, Zhang Kailu, Yin Panyue, Liu Shuhua, Zou Yuliang, Li Qiling
Department of Obstetrics and Gynecology, The First Affiliated Hospital of Xi'an Jiaotong University Xi'an 710061, China.
College of Chemistry Engineering, Tianjin University Tianjin 300350, China.
Am J Cancer Res. 2021 Jan 1;11(1):43-60. eCollection 2021.
A malignant serous effusion is one of the most common complications of advanced tumors, indicating a poor prognosis and having a profound impact on diagnosis, treatment, and prognosis. It is of great significance to identify benign and malignant effusions quickly and accurately. Both cellular and non-cellular components in the effusion can be employed for detection, diagnostic methods are necessary to obtain a definite diagnosis and more relevant information such as tumor classification. In this review, we focus on the comparison of several widespread cytological preparation methods, enrichment technology of exfoliated cells, and present tests for serous effusions, mainly including routine and special stains, immunocytochemistry, electron microscopy, enzyme-linked immunosorbent assay, flow cytometry, and molecular analysis.
恶性浆液性积液是晚期肿瘤最常见的并发症之一，预示着预后不良，对诊断、治疗及预后有深远影响。快速准确地鉴别良性和恶性积液具有重要意义。积液中的细胞成分和非细胞成分均可用于检测，需要诊断方法来获得明确诊断及更多相关信息，如肿瘤分类。在本综述中，我们重点比较了几种广泛应用的细胞学制片方法、脱落细胞富集技术以及目前针对浆液性积液的检测方法，主要包括常规染色和特殊染色、免疫细胞化学、电子显微镜检查、酶联免疫吸附测定、流式细胞术及分子分析。
