Lebedev Vladimir S, Kovalskaya Yulia, Solovyeva Evgeniya N, Zemlemerova Elena D, Bannikova Anna A, Rusin Mikhail Yu, Matrosova Vera A
Zoological Museum, Lomonosov Moscow State University, Moscow, Russia.
Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Moscow, Russia.
PeerJ. 2021 Jan 12;9:e10759. doi: 10.7717/peerj.10759. eCollection 2021.
The Tianshan birch mouse is an endemic of the Central Asian mountains and has previously been shown to include several karyomorphs ("Terskey", "Talgar", "Dzungar"); however, the taxonomic status of these forms has remained uncertain. We examined the genetic variation in based on historical DNA samples from museum collections, including the type series. Mitochondrial and nuclear data indicated that the species complex includes two major clades: Northern (N) and Southern (S) ( distance 13%). The N clade corresponds to the "Dzungar" karyomorph (Dzungar Alatau, Tarbagatay). The S clade is comprised of four lineages (S1-S4) divergent at 6-8%; the relationships among which are resolved incompletely. The S1 lineage is found in eastern Tianshan and corresponds to the nominal taxon. The S2 is distributed in central and northern Tianshan and corresponds to the "Terskey" karyomorph. The S3 is restricted to Trans-Ili Alatau and belongs to the "Talgar" karyomorph. The S4 is represented by a single specimen from southeastern Dzungar Alatau with "Talgar" karyotype. No interlineage gene flow was revealed. The validity of (equivalent to the N clade) is supported. Based on genetic and karyotypic evidence, lineages S2 and S3 are described as distinct species. The status of the S4 requires further investigation.
天山林睡鼠是中亚山区的特有物种，此前已发现它包含几种核型（“特尔斯凯”、“塔尔加尔”、“准噶尔”）；然而，这些类型的分类地位仍不确定。我们基于博物馆馆藏的历史DNA样本，包括模式系列，研究了其遗传变异。线粒体和核数据表明，该物种复合体包括两个主要分支：北方（N）和南方（S）（遗传距离为13%）。N分支对应于“准噶尔”核型（准噶尔阿拉套山、塔尔巴哈台山）。S分支由四个谱系（S1 - S4）组成，它们之间的差异为6 - 8%；其间的关系尚未完全厘清。S1谱系见于东天山，对应于指名分类单元。S2分布于天山中部和北部，对应于“特尔斯凯”核型。S3局限于伊犁阿拉套山，属于“塔尔加尔”核型。S4仅由准噶尔阿拉套山东南部的一个具有“塔尔加尔”核型的标本代表。未发现谱系间的基因流动。支持了N分支（相当于N分支）的有效性。基于遗传和核型证据，将S2和S3谱系描述为不同的物种。S4的分类地位需要进一步研究。
