Bennett Monique, Yoder Sandra, Brady Eric, Pulley Jill M, Rhoads Jillian P, Stewart Thomas G, Bernard Gordon R, Creech C Buddy, Wheeler Allison P, Thomsen Isaac
Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN, USA.
Vanderbilt Vaccine Research Program, Vanderbilt University Medical Center, Nashville, TN, USA.
iScience. 2021 Feb 19;24(2):102052. doi: 10.1016/j.isci.2021.102052. Epub 2021 Jan 9.
A detailed understanding of the adaptive host response to SARS-CoV-2 infection in humans is urgently needed. We developed a sensitive, high-throughput, and efficient assay using liquid bead array technology. We observed advantages over traditional ELISA for the detection and quantification of binding IgG against the receptor binding domain (RBD) of SARS-CoV-2. To determine whether COVID-19 symptom severity correlates with SARS-CoV-2 IgG, we measured anti-RBD IgG levels from 67 subjects recovered from PCR-confirmed COVID-19. We found that COVID-19 symptom severity strongly correlated with RBD IgG level (p < 0.001). These findings have substantial implications for public policy surrounding assessments of antibody responses and possible immunity, as not all cases of COVID-19 can be assumed to generate a protective antibody response, and mild disease in particular is capable of generating very low-level anti-RBD IgG levels. These findings also have important implications for the selection of donors for convalescent plasma to be used therapeutically.
迫切需要深入了解人类对SARS-CoV-2感染的适应性宿主反应。我们利用液滴微珠阵列技术开发了一种灵敏、高通量且高效的检测方法。我们观察到，在检测和定量针对SARS-CoV-2受体结合域（RBD）的结合IgG方面，该方法优于传统酶联免疫吸附测定（ELISA）。为了确定新冠病毒病（COVID-19）症状的严重程度是否与SARS-CoV-2 IgG相关，我们检测了67名经聚合酶链反应（PCR）确诊为COVID-19且已康复的受试者的抗RBD IgG水平。我们发现，COVID-19症状的严重程度与RBD IgG水平密切相关（p < 0.001）。这些发现对于围绕抗体反应评估和可能的免疫力的公共政策具有重大意义，因为不能假定所有COVID-19病例都会产生保护性抗体反应，特别是轻症病例产生的抗RBD IgG水平非常低。这些发现对于选择用于治疗性康复血浆的供体也具有重要意义。
