Anhui Province Key Laboratory of Chinese Medicinal Formula, School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China.
Key Laboratory of Xin'an Medicine, Ministry of Education, School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China.
J Tradit Chin Med. 2021 Feb;41(1):98-106. doi: 10.19852/j.cnki.jtcm.2021.01.012.
To explore the effects of Taohong Siwu decoction (, THSWD) on the extracellular matrix of endometrium in rats following drug-induced abortion.
Thirty-six pregnant female rats were administered mifepristone and misoprostol to induce abortion, and amounts of uterine bleeding were recorded. Pathological damage and collagen accumulation were detected by hematoxylin-eosin staining and Masson's trichrome staining in uterus, respectively. Myeloperoxidase was evaluated by immunohistochemistry.The expression levels of fibronectin, laminin, matrix metalloproteinase 9 (MMP-9), and tissue inhibitor of metalloproteinase 1 (TIMP-1) were quantified using western blotting.
THSWD could promote endometrial protection in rats following drug-induced abortion. The contents of cellulose and myeloperoxidase were significantly decreased in uterine tissue of THSWD-treated groups. Moreover, THSWD significantly decreased the expression levels of fibronectin, laminin, and TIMP-1. THSWD also significantly increased MMP-9 expression and the MMP-9/TIMP1 ratio.
THSWD plays a critical role in endometrial protection by reducing extracellular matrix deposition and uterine fibrosis. These effects may have been achieved by increasing MMP-9, reducing TIMP-1, and/or altering the ratio of MMP-9/TIMP-1.
探讨桃红四物汤对药物流产后大鼠子宫内膜细胞外基质的影响。
将 36 只妊娠雌性大鼠给予米非司酮和米索前列醇诱导流产，并记录子宫出血量。采用苏木精-伊红染色和 Masson 三色染色检测子宫的病理损伤和胶原堆积，免疫组织化学法检测髓过氧化物酶。采用 Western blot 法检测纤维连接蛋白、层粘连蛋白、基质金属蛋白酶 9（MMP-9）和金属蛋白酶组织抑制剂 1（TIMP-1）的表达水平。
桃红四物汤可促进药物流产后大鼠的子宫内膜保护。桃红四物汤治疗组的纤维素和髓过氧化物酶含量明显降低。此外，桃红四物汤还显著降低了纤维连接蛋白、层粘连蛋白和 TIMP-1 的表达水平。桃红四物汤还显著增加了 MMP-9 的表达和 MMP-9/TIMP1 的比值。
桃红四物汤通过减少细胞外基质沉积和子宫纤维化来发挥重要的子宫内膜保护作用。这些作用可能是通过增加 MMP-9、减少 TIMP-1 和/或改变 MMP-9/TIMP1 的比值来实现的。
