Zhang Yanyan, Wang Jichen, Zuo Chijing, Chen Weidong, Zhu Qian, Guo Dongdong, Wu Huanru, Wang Huizhuo, Peng Daiyin, Han Lan
School of Pharmacy, Anhui University of Chinese Medicine.
Department of Pharmacy, Anhui Medical College.
Chem Pharm Bull (Tokyo). 2018 Jul 1;66(7):708-713. doi: 10.1248/cpb.c17-00945. Epub 2018 Apr 14.
Abnormal uterine bleeding (AUB) induced by incomplete abortion is a common gynecological disease. Taohong Siwu decoction (TSD) is a traditional Chinese medicine (TCM) formula, which has been developed to treat AUB for hundreds of years. In this study, rats had incomplete abortion induced in early pregnancy using mifepristone and misoprostol. The duration and quantity of uterine bleeding were recorded and measured. The pathologic histologic grade was evaluated by hematoxylin-eosin staining (HE). Estradiol (E) and progesterone (P) levels were measured by enzyme linked immunosorbent assays (ELISA). The expression levels of estrogen receptor alpha (ERα) and progesterone receptor (PR) were detected by immunohistochemistry and Western blotting analysis. We demonstrated that TSD significantly reduced the duration and quantity of uterine bleeding. Meanwhile, TSD promoted endometrial repair and significantly up-regulated the E levels and the ERα expression. These results suggest that TSD have a protective effect on the uteri; the mechanism may be concerned with up-regulation of the levels of E and the ERα expression.
不全流产所致异常子宫出血（AUB）是一种常见的妇科疾病。桃红四物汤（TSD）是一种中药配方，已被用于治疗AUB数百年。在本研究中，使用米非司酮和米索前列醇诱导大鼠早期妊娠不全流产。记录并测量子宫出血的持续时间和出血量。通过苏木精-伊红染色（HE）评估病理组织学分级。采用酶联免疫吸附测定（ELISA）法测定雌二醇（E）和孕酮（P）水平。通过免疫组织化学和蛋白质印迹分析检测雌激素受体α（ERα）和孕酮受体（PR）的表达水平。我们证明，TSD显著缩短了子宫出血的持续时间并减少了出血量。同时，TSD促进子宫内膜修复，并显著上调E水平和ERα表达。这些结果表明，TSD对子宫具有保护作用；其机制可能与上调E水平和ERα表达有关。
